الفهرس 
REVIEW OF LITERATUREOnly 14 pages are availabe for public view
 |  | from | 132 |  |  |
| | | from | 132 | | |
Abstract
Objective – To detect and characterize PV in cats and dogs lesions and to investigate the possibility that PV cross the host barrier previously thought to exist between most species. Also, to demonstrate the rate PV associate squamous cell carcinoma (SCC) and squamous cell carcinoma insitu (BISC) in dogs and cats. Sample population – 126 cats and 22 dogs samples of various premalignant and malignant lesions (papilloma, dysplasia, SCC and BISC) were used. 10 representative samples from a total of 21 PV positive samples were selected to characterize the PVL1 gene. Procedures – Histopathological diagnosis and immunohistochemistry examination to detect PV Ag from cats and dogs samples, two primer sets (Fap59/Fap64, nested primer CP65/CP70 and CP66/CP69) were used to amplify different highly conserved region of the PV L1 gene. New PV primers were designed to amplify the entire L1 gene of the detected PV. Obtained sequences were used for molecular phylogenetic analysis and to characterize the detected virus. Results – Only one COPV was identified and sequenced out of 22 canine samples. In feline, PV was detected in 28% of cutaneous SCC and 22% of BISC, where the PV DNA was amplified and sequenced from 20 cats’ samples. 11 PV out of the 20 PV detected showed 71-99% homology to small portion of HPV L gene and 9 PV showed 96-100% homology to small portion of FdPV2 L1 gene, the entire PV L1 sequence of Two of these samples had 99% homology to the L1 gene of Human papillomavirus (HPV) type 38b subtype FA125. Another sample showed 84% homology with Human papillomavirus type 80. This PV is a new type as its sequence is less than 90% homologous to any reported L1 gene. 7 samples showed 98-100% homology to the L1 gene of recently published feline papillomavirus type 2 (FdPV2). Conclusions and clinical relevance – These data suggest the possibility of interspecies transmission of papillomavirus between humans and cats. Moreover, this is the first study to amplify the entire HPV L1 gene from cat lesions. Also, this is the first large retrospective study investigating the presence of PV in association with feline and canine premalignant cutaneous and non-cutaneous lesions, including BISC and SCC.