الفهرس 
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Abstract
Tire aim of t Ids research is the study of the effect of two drugs, the first is enrrofloxacin and the second is oxytetracycline, on the immune response of vaccinated Egyptian local breed sheep with foot and mouth disease vaccine ”01” strain.
These drugs were chosen because of their wide uses in treatment of farm animals against bacterial diseases. The two drugs were given in the legal therapeutic doses, which are 200 mg/kg h.wt intramuscular injection for enrofloxacirt and 10 mg/kg b.wt. intramuscular injection for oxytetracycline, for 3 successive days before, after and simultaneously with vaccination of foot and mouth disease vaccine ”Os”
strain. Thirty adult mixed sex sheep, 1-1.5 years old and 40-50 kg body weight, were clinically healthy and free from antibodies of foot and mouth disease. Sheep were divided into two main groups each of 15 sheep, each group subdivided into 5 groups of animals each. The groups, which used enrofloxacin drug, were as follow:
1-First group: control vaccine group only vaccinated with foot and mouth disease vaccine ”0,” strain in a dose of one milliliter subcutaneously.
2-Second group: injected intramusculrly With enrofloxacin in a dose of 200 mg/kgb.wt. for 3 successive days and after that vaccinated with foot and mouth disease vaccine.
3-Third group: injected with enrofloxacin in a dose: of 200 mg/kg b. wt. For three successive days and in the same time injected one-milliliter subcutanously with foot and mouth disease
vaccine ”0 I ”strain.
4-Fourth group: vaccinated subcutaneou sly with one milliliter of foot and tnouth disease vaccine ”01 ”strain and after 5 days injected with 200 mg/kg b.wt. intramuscularly with enrofloxacin for
3 successive days.
.5- Fifth group: were kept as virus control for the challenge test. The same divisions of animals were applied on.the other group for oxytetracyline drug.
For the study of the effect these drugs on the immune response of sheep vaccinated with foot and mouth disease ”01”strain both of cellular and humoral immune response were tested.
For cellular immune response both of total and differential leucocytic count were applied whirls revealed that it induced a significant change;” of lymphocytic count during the first three days only. On the other hand there were non significant changes in neutrophils , momocytes, eosinophil and basophil unmbers
The humoral immune response was estimated by measuring the level of serum total protein and serum albumin and globulins. Also, serum neutralization test and enzyme linked immunosorbent assay were applied for determination of the antibody titer against foot and mouth disease vaccine.
Our results revealed that there is no significant change in the Immoral immune response with administration of both the two drugs.
Challenge test were applied to measure the
potency of the vaccine in the virus control group, and revealed that the potency were 1.00% in vaccinated animals with foot and mouth disease vaccine, while there was a secondary lesions on the feet of virus control non vaccinated animals.
from this study . • It was conclude that
injection of enrofloxacin and oxytetracycline drugs in a therapeutic dose before, after and simultaneously with vaccination of foot and mouth disease ”0,” strain in sheep not affect the antibody titer resulted from vaccination. So these drugs can be used safely with foot and mouth disease vaccine in sheep.