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Abstract Fifty four water samples collected from the River Nile and its tributaries at Assiut Governorate were micro-biologically evaluated, using the total viable count, the most probable number of coliformes and Streptococcus fae-calis as well as the detection of pathogenic and pontent-ially pathogenic bacteria and fungi. It has been observed that the mean value of the total viable count per ml of the River Nile was 17.14 + 12.14 x 105 & 22.65 + 12.4 x105, while that of the Nile tributar-ies was 150.53+49.85 & 138.12+38.58 x105 at 22°C and 37°C respectively. The over all mean of the total mould and yeast count was 0.79 + 0.30 x104 per ml of the water samples at room temperature. The mean value of the most probable number of coli-form per 100 ml of the River Nile and its tributaries was 1098.75 + 127.78 and 1408.50+112.07 respectively, with an over all mean of 1270.83+83.70/100 ml. The mean value of the most probable number of faecal streptococci per 100 ml of the River Nile and Nile tribut- aries was 67.00+17.24 and 374.50+107.95 respectively; with an over all mean of 237.83+61.96/100 ml. The mean ratio of coliforms to the faecal streptoc-occi was 16.8: 1 and 3.6 : 1 for the River Nile and its tributaries respectively with an over all mean ratio of 5.3 : 1. The bacteriological examination of water samples rev-ealed the isolation of different species of pathogenic and potentially pathogenic as well as saprophytic bacter-ia. Esherichia coli (96.30%) was the commonest organism recovered from the different sources of water, followed by Streptococci faecalis (90.74%), Proteus mirabilis (70.37%), Micrococcus spp.(70.37%), Alcaligenes faecalis(66.67%) , Staphylococcus epidermidis(62.96%). Enterobacter hafniae (61.11%). Arizona hinshawii (59.26%) Citrobacter diversus (44.44%), Clostridium perfringens (42.59%), Enterobacter-Cloacae (42.59%), Klebsiella pneumoniae (40.74%), Citro- bacter freundii (37.04%), Klebsiella rhinoschleromatis (31.48%), Klebsiella ozaenae (29.63%), Proteus rettgeri (29.63%), Serratia marceseens (27.78%), Proteus vulgaris (27.78%), Achrombacter spp.(25.93%), Enterobacter liqu- ificans (25.93%), Staphylococcus aureus (24.07%), Serc- ina spp. (24.07%), Pseudomonas aeruginosa (24.07%),Enter-obacter agglomerans (22.22%)Providencia stuartii (20.37%), Alcaligenes bronchspptica (18.52%), Edwardsiella tarda (18.52%), Klebsiella aerogenes (16.67%), Enterobacter aerogenes (14.81%), Flavobacter spp. (12.96), Serratia rubidaea (12.96%), Proteus morganii (12.96%) Eikenella corradens(11.11%), Yersinae enterocoltica (11.11%), Pseudomonas alcoligens (9.26%), Acintobacter cicoac-eticus (9.26%), Shigella flexneri (9.26%), Providencia alcolifociens (9.26%), Shigella dysenteriae(7.41%), Klebsiella cloacae (7.41%), Serratia liquificans (7.41%), Morxalla spp.(7.41%), Pseudomonas putrificans (3.70%), and Alkalescens dispar (5.56%). On the other hand sal-monella failed detection in any of the samples examined. The mycological examination of water revealed the isolation of 282 fungi and yeasts belonging to 19 Genera. These fungi comprisedofllicrosporum canis I(1.8.5%), Micro-sporum distortum 1(1.85%), Microsporum oudouinii 1(1.85%), Aspergillus clavatus 6(11.11%),Aspergillus candidus 2 (3.70%), Aspergillus flavus 3(5.56%), Aspergillus funigatus 15(27.78%) Aspergillus glaucus 4(7.41%) Aspergillus nidul-ans 3(5.56%), Aspergillus niger 35(64.81%), Aspergillus terreus 2(3.70%), Madurella grisea 2(3.70%), Absidia corymbifera 2(3.70%), Absidia romosa 2(3.70%), Actinomodura spp., 2(3.70%), Actimomycis spp. 4(7.41%), Alternaria spp., 8(14.81%), Cladosporium spp. 26(48.15%), Fusorium spp. 6 (11.11%), Geotrichum spp. 1 (1.85%), Mucor spp. 8(14.81%), Paecilomyces spp. 18 (33.33%), Pencillium spp. 30(55.56%), Sepedonium spp. 9(16.67%), Trichoderma spp. 14(25.93%), Trichophyton mentographytes 2(3.7%), Trichophyton terrstre 3(3.70%), Trichophyton verrcosum 9(16.67%), non-identif- ied spp. 3(5.56%), Ceotrichum condidum 2(3.70%), Rhodotorula spp. 17(31.48%), Candida albicanis 26(48.15%) and yeast spp. 16(29.63%). It is clearly evident from our results that there is a considerable variablity of the degree of pollution from one source to another. The higher degree of pollution was recovered from El-Sohagia tributary followed by El-Tarrad tributary and El-Ebrahimia tributary. River Nile showed the lowest degree of pollution. The hygienic significance and the public as well as the animal health importance of the isolates were discussed. The justification for minimizing the risk of pollut-ion of water to an acceptable level was also discussed. |