الفهرس 
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Abstract
A total of 146 samples of luncheon (50), basterma (27),
stool from diarrhoeal children (33) and control children
(36) cases with “no complain” of diarrhoea at age range from
6 to 12 years old (whome case history was that they ate
lunchoen and/or basterma at least the day before stool
specimens collection), were collected from Gin, Cairo and
Kalyobia Governorates and examined bacteriologically for
isolation of E.coli, including the different pathogenic E.coli groups.
The incidence of E.coli in luncheon and basterma was 18%
and 11.1%, respectively, while the incidence of pathogenic
E.colj organisms in luncheon, basterma, diarrhoeal and
control children stool specimens was 8%; 3.7%; 39.4 and
33.3%, respectively. The serological identification of the isolated E.coli
revealed that out of 124 E.coli strains isolated from the
examined samples, 52 strains could be serotyped and belonged
to 15 different pathogenic E.coli serovars. These 0 serovars
were 06, OlSabOlBac, 025, 026, 028ab028ac, 044, 078, 086,
0111, Oll2abOll2ac, 0114, 0119, 0124, 0126 and 0128, those
could be categorized into 4 different pathogenic E.coli groups including enteropathogenic Eseherichia ccli (EPEC); enterotoxigenic E.coli (ETEC), enterohemorrhagic E.coli (EHEC); and enterojnvasive E.coli (EIEC).
The incidence of enteropathogenic E.coli (EPEC) serovars
in luncheon, diarrhoeal and control children cases was 6%,
30.3% and 19.4%, respectively. The incidence of enterotoxigenic E.coli (ETEC) serovars in luncheon, basterma, diarrhoeal and control children stool specimens
was 6%, 3.7%, 15.2%. and 16,7%, respectively. While the
incidence of enterohemorrhagic E.coli (EHEC) serovars in
luncheon and diarrhoeal children stool specimens was 4% and
3%, respectively; however, the incidence of enteroinvasive
E.coli (EIEC) serovars in stool of diarrhoeal and control
children was 6.1% and 5.6%, respectively. Nearly all the isolated pathogenic E.coli serovars isolated from the examined ready-to-eat meat products could
be also isolated from stool of children suffering from
diarrhoea suggesting the possibility of transmission of
these pathogenic E.coli to children through the consumption
of contaminated ready-to-eat meat products (Luncheon and
Basterma). The results revealed that the most prevalent 0 serovars
isolated from the examined ready-to-eat meat products were
078, OlSabOlBac and 0128, however, the most prevalent 0
serovars isoalted from diarrhoeal children stool specimens
were 0128, 025 and 078, while 0128 and 078 were the most
prevalent one isolated from stool of control children. The enterotoxigenic activity of the isolated E.coli that produced enterotoxin(s) was belonged to 5 serovars (Ol8ab
Ol8ac, O2BabO2Sac, 044, 078 and 0128) and also some of
untypable E.coli were LT producers. Out of 26 E.coli strains isolated from luncheon,
5(19.2%) produced LT; 2 (7.7%) produced ST and 1(3.8%)
produced both LT and ST. While out of 11 E.coli strains isolated from basterma, only one (9.1 %) was LT producer. On the other hand, out of 47 E.coli strains isolated from diarrhoeal children 10(21.3 %) produced LT; 4(8.5 %)
produced ST and 3(6.4 %) produced both LT and ST. While out of 40 E.colj strains, isolated from control children, only one (2.5 %) produced LT but non produced ST nor LT + ST. The antibiotic sensitivity test of the isolated E.coli was done using 13 different antibiotics. The incidence of antibiotic resistant E.coli against ampicillin, flumequine, chloramphenicol, carbenicillin, doxycycline, erythromydin,
gentamicin, kanamycin, nalidixic acid, novobiocin, spira
mycin, trimethoprim/sulfamethoxazole and oxytetracycline was
97.6%; 0%; 42%; 59.7%; 92.7%; 94.4%; 43.5%; 23.4%; 21%;
90.3%; 96.8%; 54.8% and 100%, respectively. It is worth
mentioning that the untypable E.coli showed more antibiotic
resistant to ampicillin , chloramphenicol, carbenicillin
kanamycin , nalidixic acid, spiramycin and trimethoprim! sulfamethoxazole than the typable pathogenic E.coli. Moreover / flumequine was considered the most effective
antibiotic against the E.coli strains isolated in the current study. The hygienic importance of the isolated pathogenic E.coli was discussed.