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العنوان
Incidence of mouds in the intestinal tract of slaughtered animals in upper Egypt in relation to meat hygiene with special reference to genus aspergillus /
المؤلف
Farghaly, Refaat Mahrnoud Mohamed.
هيئة الاعداد
باحث / رفعت محمود محمد
مشرف / على يوسف لطفى
مناقش / سعد الدين نصر
مناقش / حسنى عبد اللطيف عبد الرحمن
الموضوع
Meat hygiene.
تاريخ النشر
1985.
عدد الصفحات
95 p. :
اللغة
الإنجليزية
الدرجة
ماجستير
التخصص
البيطري
تاريخ الإجازة
1/1/1985
مكان الإجازة
جامعة بنها - كلية الطب البيطري - العلوم الطبية البيطرية
الفهرس
Only 14 pages are availabe for public view

from 95

from 95

Abstract

120 faecal samples of cattle, sheep and camel (40 samples of each) half of which was taken during the winter, while the other half in summer time from anim-als slaughtered in Assiut Abattoir. The collected sa-mples were transferred to the laboratory cit3) L mini-mum of delay, to be examined as follows :-
1. Tot al mould Co ,..1.nt :
The method recommended by Abdel-Rahnan (1981), and Klare (1971) , was applied by using acidified Malt-extract agar and Czarek-Dox-NaC1 agar media, incubated at 25°C for 7 days. Plates showing mould colonies from
20 - 200 we re chosen and counted. The total mould comet/ c.T.S. faeces was calculated and recorded.
2.Suspected mould colonies weTe isolated from the cou-ntable plates onto slope Malt agar for identification.
3.Differential count of genus jIspergillus / g. T. S. faeces was calculated and recorded.
^.
L. Cattle :
The total mould count in winter ranged from about 2000 to 400 ,000 with a mean value of about 80 1000 , while in summer these value ranged from 110,000 to 4 millions with a mean value of l.2 million, 9 mould genera could be is olated in wint er and summer in which 1,spergillus
Mucor, Lbs id ia Rhiz opus , Geotrichum, Clado- sporium, Fuzazi.u.m, Alt ernaris. and Pithonwces with the fob. flowing percentages ; ( 44. 6`/6 , 59.7) ; (3o.3% — 10.0//) ;
(5.5% — 7.5%) ; (5.4% — 7.8%) ; (4.3(/‘; — 3. 6%) ; (2.6o
5. 82,;) ; ( 2. lip — 0 9i,;) ; ( 3. 6`10 — 0,24; ) ; (1.6i Ocio) and (0 — 3 . 4`4,) respectively.
The following 10 aspergillus species co uld be id ent if ied ; A. nicer ( V. Tieghem) ; A. terre us ; A. flavus Link; A. f lave e var. co lumnetris ; A. paras it icus ; A. oryzae , A. nidulans ( Eidom) wint ; A. amstelodami; A. f um-iga tus pre sinus ; A. sydowi; A. cand id us and A. ruber
co uld be isolated in the following percent ages in winter and s amine r ; (24.9 — 42/) ; ( 27. ax, 320;-x) ; (12.4% —
13 . 2;.,) ; ( 4. 9 iL — 6.1% ) ; (0 , 4.50 ) ; (3.7, 70), ( 12.8
— 4.2 ii)) ; (3.3 — 5.8% ) ; (9.6 — 6.2%), (0.4, 0 (k) ; (0.2% — 6.1/0) and (0 , 1.7,4 re s; ectively. b. Sheep r
The total mould count ranged from 4500 to 625,000 with a mean value of about 140,000 while in summer ranged from 35,500 to 17 millions with a mean value of 2.3 mill-ions. 9 mould genera could be isolated in winter and s-summer in which the genus Aspergillus (32.7% - 56.2%) ; Penicillium (30.1io 14.4/o) ; Mucor (a/o - 7.6r0); Absidia (15`ic; - 5. 9/0) ; Rhizopu.s (2.4% - 5.3%) ; Geotrichum ( 7. 5%
- 4.4io) ; Cladosporiwn (1.510 - 1.2/0) ; Fuzarium (2.V -2.1%) ; Pithomyces could be isolated only in the summer with ( 2. 9/, ) .
The following 7 Aspergillus ppecies could be iso-lated in winter and suranpr, A. niger (44.1% - 32.1`):,)A. terreus V. Tieghem (25.8; - 13.5>” ) ; A. flavus link (14.9% - 13.7, ) ; A. flavus var. columaris - 20.2%) ; A.
nidulans (Eidom) wint (0.8% - 0.6% ) amstel oaami (3.5%
- 5.6,0) and A sydowi (1.5%- ) ; while A. oryzae, A. chevalieri and A. ruber isolated only durin4:, the winter period with the following percentages (8.9A (1.7%) and
(7.8/4 while A. parasiticus, furnigatus fres. isolated, only during the summer period with the following percentage of: (4/ ) and (6.7/0) resiectively. c. Camels
The total mould count of the examined samples in winter was ranged from about 3000 to 3009000 with a mean value of about 95,000 while in summer ranged from 84,000 to 6.3 millions with a mean value of about 1 million. 9 mould genera could be isolated in winter and summer periods with the following percentages :
Aspergillus (41% - 51.72) ; I’enicillium (21.3 - lax)) ; Mucor (3 - 7.4%) ; Absielia (16.8 - 6.3 ); Rhizopus (1 - 6.7 ; Geotrichum (3.7 - 3.7i)) ;
Llternaria: (0.7 - 2 .2;,) ; Faecilomyces (1..4 - 3.9i) and unidentified genera (0.1 io - 0.2;) while Cladosporium and Pu.zari urn could be isolated only in the winter period with percentage of (2.8A and (3.2’) respectively.
The following 8 Asp. species could be isolated in winter and suinme2_- with the followin.g. percentage of A. niter V. Tieghem (30.8(i; 38,7); A. terreus 13.3`A) ;
flavus link (12 .7k— 16.7A ; flayus varcolumnaris
(15.1/0 - a/o) ; 1)Li.YE.:Elit ICUS (2.3X, - 2.7(7,) ; A. oryzae
(10.1 - 2/0), nidulans - 3.8/, ) b.11(.1 A. candidus
(4.2;o - 3.4) I while A. fumigitus Pres. ar_d ornatus isolated only during the winter .1.:eriod with the follov,Ting percentage ( Zo) and (3.3/0) while A. ar_istela.dami; A.
sydowi and A. versicolor Tiraboschi iso fated only d urine the summer period with the percentage of (3. 9(/ ) ; ( 4.16) and (3. 4/0) respectively.
It was f wand that the cont ents of the int estinal tri.,ct of the slaughtered animals were considered as a major so urce of mould contamination of fresh meat and later on meat products. The metheds recommended to avoid such contamination were also discussed.