الفهرس 
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Abstract
Brucellosis is one of the most important diseases affecting both human and animal in most of the developing countries. It causes important economic losses Rapid and accurate diagnosis is fundamental for control and eradication of brucellosis. A total of 170 samples (90 serum samples from male and 80 serum samples from female as well as the same number from male and female whole blood samples with ( EDTA anticoagulant) at different ages and 407 serum and 407 whole blood samples with EDTA anticoagulant were collected from animals (135 cow, 55 buffaloes, 108 sheep and 109 goat ) through the period between ( December, 2008 to March, 2010 ) in different localities (El fayoum, Ebshowai , Etsa & Senorce ) at El- Fayoum Governorate .On comparing serological tests with MPCR in human (male and female ) serum and blood samples gave total positive of ELISA IgG, (32.32% ), ELISA IgM (20.58 %) , RBT (25.29%), SAT test (24.11% ) ,TAT (23.5 % ) and Multiplex PCR (32.9%). The positive results of ELISA IgG and PCR for detection of B.SPP in cow buffaloes, sheep and goat at EL-Fayoum Governorate by different serological tests and Multiplex PCR ; in cow total positive ELISA was (18.5 ), RBT (14.8%) , SAT was (14.8) TAT was (14.8) and Multiplex PCR ( 21.4% ) .For buffaloes in the same region by the same tests total positive ELISA was (14.5% ), RBT (12.7%) , SAT was 12.7%)) TAT was (12.7%) and PCR ( 5.45%) . For sheep in the same region by the same tests total positive ELISA was (28.5% ), RBT (19.44%) , SAT was ( 19.44%) TAT was (19.44%) and Multiplex PCR ( 24.7% ) and for goat in the same region by the same tests total positive ELISA was (25.68% ), RBT (26.6%) , SAT was (26.6% ) ,TAT was (26.6 %) and Multiplex PCR ( 33.03% ) . The prevalence of B.SPP in these animals may be considered the main source of Brucellosis in human through consumption of raw or under cooked meat contaminated with B.Spp or exposure to infected aborted material and discharge of infected animals with different B.Spp. ELISA proved to be simple, accurate, rapid, does not require specialized training or equipment and economical for the detection of Brucella antibody. It can be concluded that this assay could be ideal as a serological test for developing countries and rural settings, suitable for large-scale screening or presumptive test. Also, Multiplex PCR base assays were able to identify B.SPP infections followed by ELISA , RBT, followed by SAT and TAT