الفهرس | Only 14 pages are availabe for public view |
Abstract Demonstrator of Parasitology Faculty of Medicine. Ain Shams University T.vaginalis, parasitic protists of the urogenital tract, display a trophozoite and a pseudocyst stage. The pseudocyst form appears under unfavorable environmental conditions when the flagella are internalized, and a true cell wall is not formed. Pseudocysts are competent to divide but their role in trichomonas life cycle has not yet confirmed. In this study, the ability of the intra-vaginally inoculated pseudocysts to induce trichomoniasis in infected mice was evaluated in comparison to the trophozoites.Pseudocyst induction was performed physically by thermal-freezing cycle method. The infectivity of pseudocyst was proved by the presence of T. vaginalis parasite in mice vaginal washes and by histopathological studies.In vitro, T. vaginalis trophozoites and pseudocysts were found to possess several different proteinase bands by non-denaturing gelatin-SDS-PAGE (zymography). So, T. vaginalis pseudocysts are active forms that can induce trichomoniasis. Key words: Trichomonas vaginalis; pseudocysts; gelatin-SDS-PAGE; proteinase. |