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Abstract This study is concerned with the effect of three plant hormones which are salicylic acid, jasmonic acid and putrescine through their influence on the mitotic activity and their ability to cause chromosomal mutations for different time periods (3, 6 and 24 hours). It is also concerned with studying the effect of these plant hormones on the cell cycle (G1, S-phase, and G2) and changes in DNA index. The study also extended to include the effect of jasmonic acid on the various protein pattern analyzed by electrophoresis. The results showed that these plant hormones had a reducing effect on mitotic activity, leading to low rate of division and change rates in the phases of the division and especially when these hormones in high concentrations. It is evident that the use of these hormones has led to rise of many Chromosome abnormalities especially stickiness, C-metaphase, chromosome and chromatin bridges, chromosome breaks which prove the mutagenic effect of theses plant hormones. These hormones also led to changes in the cell cycle and accumulation of cells in S-phase and G2 phase. It has been noted that treatment of these hormones led to obvious changes in the protein patterns. These changes include the absence of some bands and the appearance of few novel bands as well as overexpression of the others. The results indicate the occurrence of mutagenic potentialities in the use of these hormones. |