الفهرس 
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Abstract
This work aimed to carry out a molecular and phenotypic based
characterization of APEC isolated from different f0TI11S of
colibacillosis in Egyptian poultry flocks and compare these isolates
with AFEC and environmental E. coli. Further analysis characterized
APEC associated with colisepticemia cases the most prevalent form of
colibacillosis. In addition the identification of the immunogenic
epitopes that could be used as candidates for APEC vaccine
development. Moreover this study utilized the molecular cloning as a
tool for the production of a candidate recombinant vaccine against
APEC infection.
Experiment 1: For characterization APEC isolated from different
forms of colibacillosis in Egyptian poultry flocks and compared these
isolates with AFEC and environmental E. coli. In addition further
analysis characterized APEC associated with colisepticemia cases. In
this experiment, 219 E. coli isolates from 84 poultry flocks located in
four provinces (Sharkia, Dakahlia, Gharbiyah and Ismailia) including
153 APEC, 30 avian fecal E. coli (AFEC) and 36 environmental E.
coli, were subjected to phylogenetic grouping and virulence
genotyping. Additionally, 50 of these isolates (30 APEC from
colisepticemia and 20 AFEC) were subjected to a more extensive
characterization, which included serogrouping, antimicrobial
susceptibility analysis, screening for seven intestinal E. coli virulence
genes (stxl, stx2, eae, espP, KatP, hlyA and fliCh7), multilocus.