الفهرس 
Introduction and Review of LiteratureOnly 14 pages are availabe for public view
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Abstract
Cardiovascular disease (CVD) is the primary cause of mortality and morbidity worldwide, and is projected to remain so indefinitely. In terms of global burden of disease, in 1999 the World Health Organization (WHO) placed coronary heart disease (CHD) in sixth place and stroke in seventh place, but by 2020 they will have moved to first and fourth place, respectively. CHD is the end result of accumulation of atheromatous plaques within the walls of the arteries that supply the myocardium. Recent estimates suggest that half of current healthy 40-year-old males will develop CHD in the future, and one in three healthy 40-year-old females.
In the literature of molecular genetics in cardiovascular diseases, most studies were confined to studying the frequency distribution of different genotypes in CAD patients. Some trials were performed to study the effect of the presence of a single complication in CAD patients on the frequency distribution of the different genes. However, the association of the different genotypes of some genes such as angiotensin-converting enzyme (ACE), interleukins 6 and 10 with some biochemical markers such as lipoprotein (a) and serum level of interleukin 6 as well as the impact of multiple complications on gene frequency analysis need further investigations.
The aim of this work is to investigate the gene polymorphism of angiotensin-converting enzyme (ACE), interleukin-6 -174 G/C promoter and interleukin-10 -1082 –G/A in patients with coronary heart disease as well as studying the impact of the presence of different multiple complications such as diabetes mellitus, hypertension and hyperlipidemia on the frequency distribution of different genotypes in all studied genes in CAD patients. The association of the previously mentioned genes with other biochemical markers considered to be risk factors for the incidence of CAD such as lipid profile parameters as well as lipoprotein (a) and a pro-inflammatory cytokine (Interleukin-6) was also to be tested.
To achieve the above mentioned aims, fasting blood samples were taken from subjects clinically diagnosed as CAD patients and age cross-matched healthy control subjects. Serum samples were analyzed for :
a) Glucose and lipid profile such as total cholesterol, triglyceride (TG), HDL-cholesterol, LDL-cholesterol and VLDL-cholesterol, in addition to atherogenic index,
b) Lipoprotein(a), (Lp(a)), and
c) Interleukin-6,(IL-6) by using ELISA technique,
d) DNA has been extracted from whole blood using spin columns extraction kit and PCR technique has been performed to investigate the polymorphism of the different genotypes in each studied gene (ACE, IL-6 and IL-10).