الفهرس 
Chapter I: Background and literature reviewOnly 14 pages are availabe for public view
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Abstract
The present study has covered the following points Chapter 1: (Background and literature review)
• Natural products definition, traditional medicine in ancient Egypt, anticancer drug discovery and bioassay-guided isolation strategy.
• Literature review includes short notes about plant used in the present study; Asclepias sinaica; its morphological description, chemical constituents of Asclepias through phytochemical investigation and their biological activity.
Chapter 2: (Material, apparatus and experimental) The 1st part includes material used in the present study.
The 2nd part deals with apparatus used for isolation, purification such as TLC, AGC and followed by identification of isolated compound using spectroscopic technique 1H-NMR.
The 3rd part experimental includes the following:
a. Extraction of plant material.
b. Cytotoxic activity of A. sinaica extract.
Chapter 3: (results and discussion) The extract of A. sinaica was screened against a sensitive cancer cell line, namely human lymphoma U937-GTB. The hexane extract was chromatographed on silica gel 60 using SEPARO-MPLC equipment with gradient elution running from hexane over CH2Cl2 to MeOH in increasing polarity. Eluted fractions were evaluated by TLC, combined upon similar appearance and evaporated in vacuo yielding and led to the isolation and identification of the active compound. The pure compounds are from the triterpens class and identified as Lupeol.