الفهرس 
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Abstract
This study aimed at showing the difference between patients with catheter associated UTI and those with non catheter associated UTI in terms of type of isolated bacteria , antibiotic susceptibility of isolated bacteria ,detection of their ability to form biofilm, and comparing antibiotic susceptibility of sessile cells (MBEC) and their planktonic counterpart(MIC)for biofilm forming bacteria.
In this study, urine samples were taken from fifty patients divided into two groups: First group, urine samples were taken under complete aseptic conditions with sterile syringe from distal end of urinary catheter of 23 catheterized patients (8males, 15 females) ,the second group, urine samples were taken as mid stream urine from 27 non catheterized patients (9males, 18 females) .
All samples were cultured on CLED and blood agar media and incubated aerobically at 37C° for 24 hours then the isolated organisms were identified by colonial morphology, Gram staining, and biochemical reactions.
Each group gave thirty bacterial isolates, and antibiotic sensitivity of the isolated bacteria was done using disc diffusion method, then isolated bacteria were tested for their ability to form biofilm using static MTP based system.
For the biofilm forming isolates antibiotic susceptibility of sessile cells (MBEC) were tested and compared to the (MIC) of their planktonic counterpart .For gram negative isolates Amikacin and Imipenem were used and for gram positive isolates Ciprofloxacin and Vancomycin were used.
It was found that:
1-The most frequently isolated bacteria were E-coli (31.7%) [(47.4%) in group1,(52.6%) in group2] , followed by Klebsiella (15%) [ (44.4%) in group1 ,(55.6%) in group 2] then Staph. Aureus,CoNS, (11.7%) [(42.9%) in group1 ,(57.1%) in group2] , Enterococcus (11.7%) [(71.4%) in group1, (28.6%) in group2], Proteus (10%) [(50% in group 1 and 2], Pseudomonas (6.7%)[(75%) in group1 ,(25%) in group2], and lastly Enterobacter (1.7%) single isolate in group2 .
2-The most effective antibiotics against Gram-negative isolates were found to be Imipenem and Amikacin and for gram positive isolates Vancomycin and Ciprofloxacin.
3-There was no statistical significant difference between the two groups regarding the isolated bacteria or the antibiotic susceptibility pattern.
4-Among the isolates of the first group 13 isolates out of 30 (43.3%) were biofilm forming and 17 isolates out of 30 (56.7%) were non biofilm forming
5- in the second group 9 isolates out of 30 (30%) were biofilm forming and 21 isolates out of 30 (70%) were non biofilm forming
6- The difference between two groups as regard biofilm formation was statistically non significant.
7-The organisms that were sensitive or intermediately sensitive for the antibiotic in its planktonic form became resistant in biofilm (sessile) form ,this was observed for all isolates in each used antibiotic except for tow gram negative isolates using Amikacin ,as one isolate remained sensitive and the other became intermediately sensitive.
8-The difference between MBEC and MIC for all tested strain was statistically highly significant.