الفهرس 
Stress signal transduction
RNA IsolationOnly 14 pages are availabe for public view
 |  | from | 133 |  |  |
| | | from | 133 | | |
Abstract
Differential display reverse transcription (DDRT) technique was used to detect differentially expressed genes for wild Beta vulgaris in response to salt stress. Two month-old seedlings were treated with 250 mM Nacl for 1 and 10 hr while untreated seedlings were used as controls. Differential display (DD) produced a large number of cDNA fragments, 53 of which were chosen, sequenced, analyzed using NCBI’s BLAST module and finally classified into 4 clusters according to their expression patterns (up, down, up - down, and down – up regulations). Results of the database sequence alignment identified 19 fragments with no homology in the databases, 23 fragments with homology to unknown or hypothetical ESTs with unknown functions in other organisms and 11 fragments with significant homology to genes and/or proteins with known function including Beta vulgaris chitinase and Lectin-like protein, Glycerol-3-phosphate permease like protein, phospholipase A2, ethylene forming enzyme like dioxygenase, RNA Helicase, Small GTP-binding protein, Thioredoxin H3 and Low temperature responsive glycine-rich RNA binding protein. These results implicate that several pathways are involved in the plant’s response to salt stress which still needs to be further elucidated.
Key words: salt stress, cDNA, Differential display (DD-RT), Gene expression, Beta vulgaris.