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Abstract
Does Methionine Supplementation
Alters Beta Amyloid Levels in Brain Cells, Liver and kidney Functions?
Nada Ahmed Abd El Aty,
B.Sc. Home Economics, 2008 Nutrition and Food Science Department, Faculty of Home Economics Helwan University
;4.6stract
The present study was carried out to investigate the effect of methionine supplementation on beta amyloid levels in brain cells, liver and kidney functions. Fifty adult male of Spargue - Dawley strain rats, weighing 160 ± 5 gm were used. Rats were divided into 5 groups, (10 each) the control negative group was fed on basal diet, while the control positive was fed on basal diet with substitution of casein with methionine. The other groups from 3 to 5 were fed the basal diet supplemented with 1 %,2% and 4% methionine. Results of this study revelated that feeding diet with methionine at the highest two levels had significant increase in serum levels of beta amyloid (60.42 ± 8.32 Nlml and 72.36 ± 12.06 N/ml, respectively) compared to the positive control group (53.66 ± 8.52 N/ml). Also, when rats were fed diet with methionine at 4% level lead to significant increase in the level of beta amyloid concentration in brain (2.57 ± 0.35 N/gm) compared with either control negative group (1.04 ± 0.17 N/gm) or control positive group (1.73 ± 0.21 N/gm). However feeding methionine at concentrations of 1 % and 4% caused significant reduction in serum levels of GPT (13.05 ± 7.36Vlm and 23.06 ± 5.82 Vim, respectively), whereas, 2% methionine had no significant change (30.44 ± 2.93 Vim) compared to the positive control group (27.95 ± 8.62 Vim).
Feeding methionine at the tested three levels caused significant reduction in serum levels of GOT (37.96 ± 10.64 U/m, 5196 ± 4.11 U/m and 48.44 ± 8.87 U/m, respectively) compared to rats fed on 15% methionine (positive control group). Concerning uric acid concentration in the blood, rats fed methionine in the diet showed significant decrease when consumed at any level of intake (2.92 ± 0.12 Mg/dl, 2.39 ± 0.10 Mg/dl and 2.79 ± 0.03 Mg/dl, respectively) compared to the control positive group (2.94 ± 0.19 Mg/dl). Methionine ingestion at the three concentrations (1 %, 2% and 4%) caused significant reduction in serum levels of urea nitrogen (17.71 ± 0.18 Mg/dl, 13.69 ± 0.18 Mg/dl and 18.18 ± 0.33 Mg/dl, respectively) compared to the positive control group (23.61 ± 0.41 Mg/dl). This study suggests that protein consumption should be upon recommendation since it has deleterious effects on organs and brain nutrition education program should be carried out to the public.
Key words: Beta Amyloid, Methionine, Glutathion Peroxidase, Brain, Liver, Kidney and Rats.