الفهرس 
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Abstract
This work was carried out at the Plant Tissue Culture Laboratory in Faculty of Environmental Agricultural Sciences (FEAS), El-Arish, Suez Canal University (SCU) during the period from 2006 to 2009.The objective of this study to establish an applicable protocol to save the endangered native Egyptian plants, Capparis spinosa L., Capparis cartilaginea Decne and Solenostemma arghel Del. through In vitro micropropagation. The results showed that the Murashige and Skoog (MS) was found to be the best medium and one node cutting as starting materials for establishment stage for mother plants. Multiple shoots were obtained on MS medium supplemented with 0.50 mgl-1 6-benzyladenine (BA) in the combination with 0.05 mgl-1 α-naphthaleneacetic acid (NAA). The elongated shoots achieved with MS medium supplemented with a combination of 0.5mgl-1 BA with 0.05 mgl-1 NAA plus 0.5mgl-1 GA3. After that, rooting the shoots on ½ MS semi solid +1.5, 1 and 2 mgl-1 IBA alone or with 2 gml-1 AC for C. spinosa, C. cartilaginea and S. argel. Finally hardening the rooted shoots in greenhouse in pots containing mixture of peat-moss and vermiculite (1:1v/v) for C. spinosa, C. cartilaginea and peat-moss. vermiculite and sand (1:1:1). Regenerated plantlets were successfully acclimatized with 90% survival for C. spinosa and C. cartilaginea while 93% survival for Solenostemma arghel .
Keywords: Capparis spinosa, Capparis cartilaginea. and Solenostemma arghel Micropropgation, nodal, BA, NAA , IBA Survival rate and Activated Charcoal.