الفهرس 
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Abstract
This study was carried out in In vitro Biology and Horticulture Unit,
Faculty of Bioscience Engineering, Ghent University, Belgium during the
period from 2009 to 2011.
The aim of this study was to establish and develop protocols for in vitro
propagation of non-toxic Jatropha eureas lines (Kenyan and Mexican)
through different culture techniques (axillary bud, shoot tip proliferation and
direct adventitious shoot bud regeneration from leaf discs).
The most important results obtained from this study can be summarized
as follows:
A- Culture establishment stage (Shoot Induction Stage):
1- The highest number of shoots/explant, shoot length and number of
leaves were recorded on Murashige and Skoog’s medium basal salt
mixtures including vitamins (MS) supplemented with 3% sucrose,
0.8% Agar and 1.0 mg/l Benzyladenine (BA) without callus formation.
2- Increasing the concentration of BA up to 1.0 mg/l decrease shoot
regeneration of Jatropha eureas plants.
3- Using Thidiazuron (TDZ) individual as a cytokinin with different
concentrations did not give good results for in vitro propagation of
Jatropha curces but we can use it for induction of callus.
B- Shoot multiplication stage:
Influence of PGRs and media composition:
1- The highest results of the most growth parameters were obtained with
Murashige and Skoog’s medium basal salt mixtures including vitamins
(MS) then Gamborg medium (B5).