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Abstract Twenty eight soil samples were collected from vanous sites (road soils 11, fertile soils 11 and farm soils 6) and was subjected for mycological examination to evaluate the presence of geophilic dermatophytes in Sharkia Governorate. By using modified hair-bait technique, 32 keratinophilic fungal isolates were obtained from 24 soil samples while 4 samples were negative. The isolated keratinophilic fungi were identified into Mgypseum (64.28%), Tmentagrophytes (10.71%) and C.keratinophilum (39.28%) out of28 soil samples. Concerning the effect of different hair as a source for keratinous substances in modified hair-bait technique. It was found that horse hair and goat hair were more effective since it facilitate the isolation of keratinophilic fungi from 24 samples (100%) out of 24 soil samples while cattle hair, sheep wool and feather from birds gave positive culture from 22 (91.66%), 21 (87.5%) and 20 (83.33%) out of24 soil samples respectively. Eighteen isolates of Mgypseum obtained in this work were subjected for identification by macro and micro-morphological characters as well as using of differential media. |