الفهرس 
Statin - induced myopathyOnly 14 pages are availabe for public view
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Abstract
Benefits of statins in management of hypercholesterolemia might be restricted by its myopathic side effects. A promising approach is constituted by stem cell therapy which is used as regenerative agent. In accordance with this, this work was to study the picture of simvastatin-induced myopathy and to evaluate the effect of bone marrow derived mesenchymal stem cells on simvastatin-induced myopathy Ninety adult female rats were used in this study; ten of which were used for isolation and preparation of MSCs. The other rats were divided randomly into four groups: control; simvastatin induced myopathy; recovery and MSCs treated group The Control group was further subdivided into –ve and + control sub groups, while the recovery and MSCs treated groups were subdivided into two subgroups according to time of their scarification at 15 and 30 days. Simvastatin was administrated at a dose of 80 mg/kg b.w /day as a single oral dose daily for 30 days for groups II, III and IV, while MSCs was administrated as single intravenous injection, via rat tail vein at a dose of 6x106 cells. Clinical signs and weight of rats were recorded every 15 days all through the experiment and the percent of change were calculated. At the time of scarification, rats were anaesthetized and blood samples were collected for biochemical analysis. Gastrocnemius muscle samples were dissected for histological; histochemical and immunohistochemical studies, the later was for myosin and desmin detection. Also, morphometric measurements were done using image analyzer and homing of PKH26 labeled MSCs was detected by fluorescent microscope. In this study, there was no significant difference detected between control subgroups regarding clinical; biochemical; histological and
immunehistochemical studies. Simvastatin- induced myopathy group showed adverse physical signs, such as a decrease in food consumption and a decrease in body weight and an elevation of serum CK level up to 3.5 folds. Muscle of this group showed variability of fibers size; splitting; fragmentation; vacuolation and cellular infiltration. In addition, histochemical and immmunohistochemical stains showed weak reaction. In comparison with myopathic group, recovery group showed no improvement with worseness of myopathic picture, while MSCs treated group showed a significant improvement of its myopathic picture and for both groups the results were time dependent. All this data was based on clinical; biochemical; histological ; histochemical and immunohistochimical studies.