الفهرس 
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Abstract
In these thesis serum and skin lesions were collected from camel suffered from camel pox virus (CPV) infection from different region of South Sina Governorate and Maruit camel farm of the Desert Research Center, serum neutrization test (SNT) applied on the collected serum sample resulted in presence of specific antibody against CPV that ensure the causative agent of the infection is CPV,
CPV was isolated and propagated on CAM of 9-11days SPF–ECE for 10 passages resulting in characteristic pock lesion of the CPV and the highest titer was (4.2 log10TICD50/ml) at the 7th passage, also CPV was isolated and propagated on Vero cell line for 20 passages resulting in the characteristic CPE in the passage no3 after 5days of inoculation and the virus titer increased gradually till reach (4.7 log10TICD50/ml) at the 17th passage. The antigenic characterization done on the isolated virus on Vero cell line using SNT and DAS-ELISA on Ag prepared from different propagated isolated virus passages revealed that the isolated virus is CPV. Multiple aliment based on sequence analysis for PCR amplification for the C18L gene of CPV isolated and propagated on Vero, extracted from crust sample and vicinal strain result in clustering the isolated Egyptian CPV with reported CPV isolates in gene bank with identity reaching 100% on the level of nucleotide and amino acid while the CPV vaccine is clustered with vaccinia virus. Key words(PCR-sequence analysis-C18L-ATIP-CPV).