الفهرس 
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Abstract
The present study was carried out on a total of 85 cases presenting with symptoms
that might be suggestive of intestinal giardiasis. All stool samples were subjected
to: a) microscopical examination using direct wet smear, concentration technique
and Iron Hematoxylin (Fe.Hx) staining. b) Direct immnnofuorescent assay (DFA).
c) DNA extraction followed by a PCR, using nested primers specific for Giardia.
Considering the nested PCR (nPCR) as the gold standard test, sensitivity of stool
microscopy using the direct examination and concentration technique and that of
stool microscopy using permanent Iron Hematoxylin stain was found to be 66.7%
and 72.9% respectively with a statistically significant difference. While the
sensitivity of DFA was 85.4% in detection of Giardia infection with a statistically
significant difference compared with the nested PCR. No false positive results
were recorded; therefore, specificity for all tests used was 100%