الفهرس 
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Abstract
Aim: There is a need to investigate the effects of using low intensity laser irradiation on SHED proliferation, mineralization, and scaffold degradation as part of future regenerative endodontic procedures. Methods: Isolation and preparation of stem cells was carried out from SHED using enzyme-digestion method. Characterization of SHED was done by using flow cytometry at passage 5. The effect of laser phototherapy on SHED and collagen scaffolds was investigated using GaAlAs (830 nm) low intensity laser with varying irradiation times. Results: The flow cytometric analysis showed that SHED to be positive to the markers of CD73 and CD105 while they were negative to CD34 marker. LILI energy density and irradiation times could enhance or reduce SHED proliferation. All the cell cultures showed ascending mineralization percentages from 14−28 days. Conclusions: Laser phototherapy can influence SHED differentiation, leading to improved regenerative endodontics.