الفهرس 
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Abstract
•In this work, some bacteriological and immunological studies were carried out on E. coli and S. aureus strains for evaluation of the effect of ~- lactam antibiotics treatment on the bacterial
susceptibility to Phagocytic cell activity.
•At first, 4 typed bacterial strains of E. coli and 4 typed bacterial
strains of S. aureus isolated from clinical cases were used as the
test organisms. They were cultivated on MacConkey’s agar and Nutrient agar with (7-10%) NaCI, respectively.
•Disc diffusion antimicrobial susceptibility test was done by
using all strains then amoxicillin and piperacillin were chosen as
examples for ~- lactam antibiotics to be used for treatement of E.
coli and S. aureus bacterial strains predisposing to undergo some
immunological studies on them.
•E. coli and S. aureus strains were treated with amoxicillin by 18
hs incubation with 1/3 (MIC) value which recorded (8 ug/ml)
for E. coli and (4 ug/ml) for S. aureus•E. coli and S. aureus strains were treated with piperacillin and
this achieved by picking up the arisen colonies in the middle zone around Piperacillin disc on disc diffusion sensitivity plate.
•Concerning culturing of peripheral blood mononuclear cells
after its isolation from sheep jugular vein puncture which was a
simple reproducible method for obtaining a high yield of viable
phagocytic cells, assessment of cell viability and counting of the
cells and adjustment the count of the cells, then the amoxicillin
and piperacillin treated E. coli and S. aureus cells and untreated