الفهرس 
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Abstract
-Paper electrophoresis was used in the separation of Phaseolus vulgaris proteins.
Electrophoretic analysis showed no variation in the n~nber of the bands located in the electrophoretic patterns of the three varieties examined.
-Modified methods were carried out in order to separate each protein.
-studing the proximate composition and nutritive value of Phaseolus vulgaris varieties, contender; swissblan; and giza (local varieties ).
-Determination of amino acids in the isolated fractions, albumin ; globulin A ; and globulin B.
-Determination of total nitrogen in every fraction.
-Studying the extractability of nitrogenous constituent
in the three variety.
·The densitometer were used for quantitative determinaation of the soluble Phaseolus proteins.
·The swissblan variety contained relatively higher protein than other two varieties.
(ii) Lysine content of the Phaseolus seeds is relatively -1
high, its amount ran~ed 8.05 to 8.63 g/16g N.
(iii)Tryptophan was slightly higher for giza variety than
tyros . .ine on.ly d.isClppear .in the c.lJromBtOt78.7J oj’ 61 D~~uJin A. The analysis of globulin B gave all the
( v ) Globulin A differs chiefly from the globulin B in having less nitrogen and a much higher sulfur amino acids content.
(vi) The amount of aspartic acid is increased with a decrease in glutamic acid in all the analyzed fractions.
(vii) from the analysis of the free illnino acids contents fourteen amino acids appeared in the chromatogram
of the three varieties two of t~em are not idenntified. The amino acids cystine, phenyl-alanine
and tyrosine were absent in the extract of the
three varieties. The highest levels of free a~ino acids were reported for contender variety and swisssblan.
- Studing on the effect of different salts on the extracttion of nitrogenous compound showed that the amount of nitrogen extracted can be arranged on a decreasing
order for every variety as follows swissblan, conntender; -and giza and this agree vIi th the amount of total nitrogen, 25.1 % ; 2).8 % ; and 21.45 % respectively.
The present findings emphasize the importance of supplementing other cereals with Phaseolus vulgaris
for its high protein content and high caloric value.