![]() | Only 14 pages are availabe for public view |
Abstract presence of spenns by observing the specimens using light microscope . This day was delennined to be day of pregnancy. After pregnancy, the control females and the experimental females were divided into three subgroups, five females each: Subgroup (A); 5 pregnant mice sacrificed on the 18th day postcoitum by utilizing ether inhalation technique . The abdomen of each animal was incised , the left and right uterine horns were opened, and the fetuses were removed and decapitated using sharp blade. Subgroup (B); the neonates of 5 pregnant mice were obtained immediately at birth. Subgroup (C); the offsprings, were obtained two weeks after birth . The heads of the fetuses of the control and experimental animals were obtained and fixed in 10% neutral buffered fonnalin . Further processing and embedding in paraffin were carried out. 6 microns thickness sections were obtained and stained by routine hematoxylin and eosin stain for histological interpretation. For immunohistochamical study we us In group I (control group) hematoxylin and eosin stained sections showed nonnal tooth development in all periods. In group II (experimental group ): Group II(A), there were obvious histological changes in comparison with the control group. These histological changes include: (I)retardation in development, the molar tooth genus were in late cap stage ,while in control in early bell stage. (2) presence of inflammatory cells. (3) poorly organized enamel organ and dental papilla with obvious cystic - degeneration. (4)poorly developed dental sac as well as bone trabeculae. |