الفهرس | Only 14 pages are availabe for public view |
Abstract Plenty amount of bacterial genomic data provides unparalleled opportunities for understanding the genetic and molecular bases of the degradation of aromatic compounds. In this study 206 extradiol dioxygenases type I (Edos) genes were collected, but only sixty-eight genes were selected as aromatic compounds degrader. Sphingomonas wittichii RW1, which is the only strain that can mineralize dibenzo-pdioxin and its chlorinated derivatives. Study showed that at least three new different extradiol dioxygenases type I genes are encoded in the RW1 genome, namely edo9, edo10 and edo11. The three Edos genes were amplified by PCR, cloned into pTz57R/T vector, and over expression was detected by SDS PAGE. These findings may benefit in the bioremediation of dioxin. |