الفهرس 
1- IntroductionOnly 14 pages are availabe for public view
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Abstract
The importance of early diagnosis of bovine tuberculosis is due to
its widely spread in the world especially in the developing countries. It is
also highly zoonotic disease between animal and human.
The present study was done on 900 animals (480 buffaloes and 420
cattle) from different localities at Minufiya and Gharbia governorates
within three years (from 2009 to 2011). By application of tuberculin test
on these animals, 9 cattle (2.14 %) and 27 buffaloes (5.62 %) were
positive reactors while the remaining animals were negative. These
positive tuberculin tested animals were slaughtered and examined to
determine the post mortem lesions, 8 out of 9 cattles (88.9%) and 4 out of
27 buffaloes (14.8%) showed visible lesions.
Comparing the rate of infection between examined animals with
the age, found that, the highest rate of infection was in age (4-6) years
and the lowest rate of infection was at young age less than 2 years.
The relationship between rate of infection and P.M. finding in
cattle showed that in young age up to 2 years there is no visible P.M.
lesion, and the highest tuberculin positive ages were (4-6) years, showed
that P.M. lesions were 4 visible lesion (2 cases of them Pulmonary lesion,
1 case digestive and 1 case mixed P.M. lesion, and not showed
generalized lesion. Also in buffaloes, the highest tuberculin positive ages
were (4-6) years and the P.M. lesion was 1 visible lesion (digestive) and 9
non visible lesions.
Summary
110
The lymph nodes and tissue samples were collected from the
positive tuberculin (9) cattles and (27) buffaloes, cut with sterile scalpel
and divided into 3 categories:-
1- The first part was used for histopathology and Ziehl Neelsen
staining.
By histopathology and Z.N. staining of acid fast bacilli of positive
tuberculin reactors tissues, found that all 9 (100%) tissues of positive
tuberculin cattle gave positive when stained with Z.N., also they were
positive with H&E. While from 27 positive tuberculin buffaloes,
5(18.5%) tissues gave positive with Z.N. and 17(62.96%) tissues gave
positive with H&E.
2- The second part was used for culture and Ziehl Neelsen staining of
the isolates then LCD array and PCR on the isolates.
The bacteriological examination of 9 tuberculin positive cattle
tissues (8 with VL and 1 with NVL), 8 (88.9%) acid fast bacilli were
isolated. The total 8 tissues showed visible lesions grew on Lowenstein-
Jensen medium with sodium pyruvate and all the 8 isolates (100%) gave
positive with ZN stain.
PCR & LCD Hybridization array were applied for detection of
Mycobacterium tuberculosis complex in the 8 positive isolates. The result
was (100%).
We confirmed the positive results in 4 isolates with PCR test using
primers which confirmed the presence of M.bovis.
Summary
111.