الفهرس 
CHAPTER I: INTRODUCTIONOnly 14 pages are availabe for public view
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Abstract
A literature survey was presented on ornidazole, secnidazole and tinidazole
containing their structure, biological activity, microdetermination and separation at
various condition using different reagents. Also literature survey on phydroxybenzaldhyde
concerning its chemical, physical properties, its reaction and
application
(2)Chapter II:-
Simple, rapid, precise and reliable methods were for the microdetermination of
ornidazole, secnidazole and tinidazole.
a-the first part: - included spectrophotometric determination of ornidazole,
secnidazole and tinidazole through Schiff’s base reaction with phydroxybenzaldhyde.
1- The coloured Schiff’s base were prepared using p-hydroxybenzaldhyde and
measured at selected λ max for each drugs.
2- The optimum ratio between reactant was found to be 1:2 (drug: phydroxybenzaldhyde)
3- 50 O C was chosen as the best temperature as the effect of the
temperature on the reaction
4- The extent of the reaction with p-hydroxybenzaldhyde increasing
generally as a function of time and tends to equilibrium after 30
minutes for all drugs.
5- In microdetermination of ornidazole, secnidazole and tinidazole with
p- hydroxybenzaldhyde, Beer’s law was valid in the concentration range between of
20-300 μg ml-1 for ornidazole, secnidazole and 20-250 μg ml-1 for drug tinidazole.
6- Pharmaceutical preparations of the cited drugs supplied from different
companies were determined spectroscopy using p-hydroxybenzaldhyde reagent. The
results obtain by the suggested procedure were compared with those obtained by
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standard methods. The data obtained by both procedures found to be very close to
each other and very close to those given by the Pharmaceutical companies.
b- the second part: Cyclic voltammetry and differential pulse voltammetry were
used to explore the adsorption behavior of the drugs under investigation ornidazole,
secnidazole and tinidazole at activated glassy carbon electrode, and well defined
reduction peak was obtained in Britton-Robinson buffer of pH 7 for the cited drugs.
The adsorption response was evaluated as a function of some variables such as the
scan rat, pH and accumulation time and potential. The voltammetric method was
found to be simple, accurate, inexpensive and sensitive for the determination of the
cited drugs. A linear calibration was obtained from 2X10-9 to 4X10-7M for
ornidazole, 2X10-9 to 1X10-7M for secnidazole and 2X10-9 to 6X10-7M for tinidazole.
Calculated limit of detection (LOD) values were 2.5X10-10, 2.0X10-10 and 3.0X10-
10M while calculated limit of quantification (LOQ) values were 4.4x10-8, 4.0x10-8 and
1.2X10-8 M for ornidazole, secnidazole and tinidazole respectively. The RSD values
of five measurements were 0.37, 0.5 and 0.66 for ornidazole, secnidazole and
tinidazole respectively. The method was applied to the determination of the
investigated drugs in row materials and pharmaceutical preparations and the results
obtained were compared with those obtained from the official methods.
The thesis was finished with a number of references which help the explanation and
comment on the results.
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