الفهرس 
General properties of MycoplasmaOnly 14 pages are availabe for public view
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Abstract
Summary and Conclusions The aim of this work is to study the role of M.hominis and U. urealyticum infections in SLE and RA by diagnosis both infections in urine bacteriologically and determination of antibiotic susceptibility pattern of them. Early morning urine samples were obtained from 40 SLE and 40RA female patients and 20 healthy women as control group. The age of the studied RA and SLE groups was ranged from 22 to 58 years while the age of controls was ranged from 25 to 40 years. The collected urine samples were examined for the diagnosis of U.urealyticum and M.hominis and detection of their antibiotic susceptibility tests by using:
Ι- Urée – Arginine LYO 2(Biomerieux, France) as screening test for mycoplasma
ΙΙ- Mycoplasma IST2 Kits (Biomerieux, France) for cases in screening test to diagnose, detect the mycoplasma species and their antibiotic susceptibility to different antibiotics.
By comparing the two culture methods used in this study it was found that by Urée – Arginine LYO 2:27 (67.5%) cases, 24 (60%) cases and 5 (25%) of RA, SLE and control subjects respectively were positive for mycoplasma infection. While by Mycoplasma IST2 Kits it was found that out of 40 RA patients: 17 (42.5%), 10 (25%) and 6 (15%) had U.urealyticum, M.hominis and both infections respectively. Out of 40 SLE patients: 15 (37.5%), 9 (22.5%) and 5 (12.5%) had U.urealyticum, M.hominis and both infections respectively. Four (20%) and 1 (5%) of 20 control subjects had U.urealyticum, M.hominis respectively. The titer of mycoplasma was < 104 in control group which means mycoplasma colonization not infection.
Among SLE and RA patients, the age had insignificant statistical value with culture results. In RA patients the type of mycoplasma infection either M.hominis or mixed U.urealyticum and M.hominis had insignificant statistical value with the disease activity, while U.urealyticum infection had a significant statistical value with the disease activity. In SLE patients U.urealyticum or mixed U.urealyticum and M.hominis had insignificant statistical value with the disease activity. While; M.hominis infection had significant statistical value with the disease activity.
As regards the antibiotic susceptibility tests for the diagnosed mycoplasma cases, it was found that josamycin 8mg/L and pristinamycin 2mg/L were the most effective antibiotics with nearly 100% response for all strains. Doxycycline 8mg/L was 100% effective for M.hominis and erythromycin 4mg/L was 100% for U.urealyticum. Tetracycline 8mg/L is effective with (80% to 100%) response for both U.ureaplasma and M.hominis. The results also indicate that josamycin, pristinamycin, doxycycline and tetracycline are the first choice drugs when empirical therapy is required.
conclusions:
Mycoplasma infection had a role in SLE and RA diseases. So the detection of mycoplasma remains important and necessary because of its involvement in several urogenital and autoimmune diseases. As the cultivation of mycoplasma is laborious, time consuming, requires specific experience; Urée – Arginine LYO 2 is a suitable method for screening of mycoplasma, easy to be use and the results were available in 48hs although it can’t differentiate between the different species of mycoplasma. Mycoplasma IST2 Kits (Biomerieux, France) have a valuable role for the diagnosis of the type of mycoplasma infection and determination of its antibiotic susceptibility tests.