![]() | Only 14 pages are availabe for public view |
Abstract Legume seeds are considered a main source of protein for human and animal nutrition. Bruchids are serious pests and cause considerable damage to seeds. One–year survey was carried out to study the prevalence of bruchid insect pests and its associated parasitoids encountered in stored leguminous seeds residues. The effects of each of crowding, interspecific competition and host seeds on some biological parameters of the most two frequently encountered species, i.e. Callosobruchus maculatus (Fab.) and Callosobruchus chinensis (L.) were also carried out. RAPD-PCR-based fingerprinting of four bruchid pests and its three associated parasitoids was also conducted. The obtained results can be summarized in the following points: 6.1 Survey and population fluctuations of stored leguminous seeds insect pests.… …………………………………………………………... 6.1.1. Survey of stored leguminous seeds insect pests A total of 552 adult individuals (per 12 kilograms) representing five different insect pest species belonging to Bruchidae, order Coleoptera were counted from the residue samples collected from the seed warehouses located at El-Sahel stores, during 12 months of survey work. The five bruchid insect species detected were: Callosobruchus maculatus (Fab.), Callosobruchus chinensis (L), Callosobruchus quadrimaculatus (F.), Bruchidius incarnatus (Boheman) and Acanthoscelides obtectus (Say). Generally, the lowest number of adult insects detected in the bimonthly seed residue samples was during January, where the mean daily temperatures ranged between 13.10 - 15.60ºC and 63.00- 69.00 % R.H. Meanwhile, higher number of insects was found in summer months where temperature was high. Bearing in mind that this survey was only concerned with the adult stage of each insect species. from the conducted survey, the encountered coleopteran species Callosobruchus maculatus and Callosobruchus chinensis presented the highest percentage of collected insects 38.76 and 26.08 %, respectively. These were followed by a lower percentage of 15.57 and 13.04% for Callosobruchus quadrimaculatus and Bruchidius incarnatus, respectively. Meanwhile, Acanthoscelides obtectus were presented by 6.52%... Monthly total percentages of the numbers of five coleopteran insect species collected from leguminous seeds residues were higher in summer season 17.93, 13.04 and 16.48 % for August 2006 and June and July 2007, respectively. Winter months were the lowest percentages being 2.35, 0.54 and 1.63 % for December 2006, January and February . |