الفهرس 
Acute Myeloid LeukemiaOnly 14 pages are availabe for public view
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Abstract
In this study, we assessed each of CD123 (Interleukin-3 receptor alpha chain) & NF-κB (nuclear factor kappa B) in primary adult de novo AML patients and the relation between them at diagnosis and at follow up .
CD123 expression was achieved through Immunophenotyping by flow cytometry (FCM) on BM aspirate and quantitation of NF-κB in BM samples using ELISA were performed for the following levels: plasma, cytoplasmic and nuclear fraction of blasts, as well as cytoplasmic / nuclear (C/N) ratio was calculated.
At diagnosis, CD123 was positive for all cases of de novo AML and the median percent of cells expressing CD123 was (54.1±24.7%) and when CD123 expression at time of diagnosis was compared between remitted & resistant patients , we found a significantly difference of expressing CD123 being higher in resistant group .
At follow up, there was a decrease in CD123 expression after therapy, the level of CD123 expression decrease from (54.1±24.7%) to (36.5±24.8%) and when CD123 expression at D28 on bone marrow we found a significant higher expression of CD123 in resistant patients compared to remitted patients .
Regarding, NF-κB at diagnosis, NF-κB was found significantly higher in de novo AML patients compared to control subjects [plasma (1533.5±2258.6.7 pg/ml VS 674.5±420.6pg/ml) – cytoplasmic fraction (2103.0±1417.5 pg/ml VS 1632.5±518.8pg/ml) – nuclear fraction (1588.6±1149.3pg/ml VS 806.8±195.3pg/ml)- C/N ratio (1.70±1.43 VS 2.03±0.79)]
As the same for CD123, at follow up, we found significant decrease in NF-κB levels detection after therapy in (plasma- cytoplasmic fraction – nuclear fraction – C/N ratio). The levels of NF-κB detection decreased from (1533.5±2258.6 to 706.5±264.1 pg/ml)- (2103.0±1417.5 to 803.8±349.1 pg/ml )-(1588.6±1149.3 to 755.5±360.0 pg/ml)-(1.70±1.43 to 1.09 ±0.36) respectively at D28 follow up by bone marrow examination.
Interestingly ,when NF-κB levels in (plasma- cytoplasmic fraction – nuclear fraction) were detected at follow up (D28) on bone marrow expression , we found also a significant higher levels of NF-κB in resistant patients compared to remitted patients (876.3±268.5 pg/ml VS 593.3±199.2 pg/ml) (p=0.021) - (1020.0±177.0 pg/ml VS 659.6±366.0 pg/ml) (p=0.031) - (948.8±305.2 pg/ml VS 626.7±345.4 pg/ml) (p=0.006) respectively .
Which was in parallel with CD123 findings but the only exception was regarding C/N ratio was not significantly different (1.12±0.21 VS 1.08±0.44) (p=0.440)
Although , throw our study, we found a significant positive correlation between Uric acid and both of CD123 and NF-κB, leading that uric acid is a common finding and we hypothesized that it could be considered as a prognostic factor .
In the relation between CD123 and NF-κB levels in de novo AML we established a positive correlation between CD123 & only NF-κB plasma level at time of follow up but we were not succeeded to establish this correlation at time of diagnosis.