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Abstract Salmonella infections in farm animals have been recorded in most countries of the world. Diarrhoea and deaths in farm animals is the main cause of economic losses in the world. Therefore the aim of this work was planned to discuss the isolation and identification of the causative agent. Serological typing of isolated Salmonella strains. Use ofPCR technique to confirm the isolates, application of RAPD-PCR to detect variation between species and determination of antibiogram of Salmonella isolates. A total of 770 rectal swabs were collected from farm animals,90,80,100 from apparently healthy cattle, sheep and goats respectively and 150,140 and 210 from diarrhoeic cattle, sheep and goats. Bacteriological finding of examined cases from apparently healthy cattle, sheep and goats revealed that 5,3 and 2 out of 90,80 and 100 faecal samples were bacteriologically positive for Salmonella with incidence of 5.6%, 3.75% and 2% respectively, while, the examined diarrhoeic cattle, sheep and goats revealed that 22, 13 and 16 out of 150, 140 and 210 faecal samples were Salmonella positive with an incidence of 14.7% , 9.2% and 7.6% respectively. |