الفهرس 
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Abstract
Neonatal calf diarrhoea remains one of the most common and economically devasting diseases of cattle industry specially when occurred in the first weeks of their life (in calves under 30 days of age).
Rota and corona viruses are considered the most common viral agent associated with this syndrome.
As the hazard of the calf loss by RV and CV infections is usually awaited during the first few days of life and also the protection against both viruses is basically depend on the presence of adequate of specific antibodies in the gut lumen of newlyborn calves, the correct protection program should be directed to active immunization of pregnant darns during the last stage of pregnancy.
The present study was planned for preparation and evaluation of combined inactivated vaccine from BRV and BCV using alhydrogel and oil (Montanide ISA206, Montanide IMS 1113) as adjuvants and compared these oil adjuvants with the gel adjuvant vaccine.
The evaluation of these prepared combined inactivated vaccines was conducted in laboratory as follow: Sterility and purity tests, where the obtained results proved that the vaccines were pure and free from any viable micro organism (viral, bacterial, mycroplasma and fungal).
Safety tests was performed on laboratory animals (mice and guinea pigs) and calves and the results revealed that there was no abnormalities or deaths among the laboratory animals or elevation of body temperature and appearance of any clinical abnormalities in calves during the observation
period.
So the result indicates the safety of the prepared vaccines:
Potency tests were conducted in laboratory on mice and calves and in field on pregnant cows in late stage of pregnancy and their offsprings.
Different tested animals were divided into four groups which three of them inoculated by gel adjuvanted vaccine and oil adjuvanted vaccines (ISA 206 and IMS 1113) respectively and the fourth group kept unvaccinated as
control.
Two serological experiments (SNT and ELISA) are developed to estimate the immune response against the two viruses.
The two tests were conducted on the sera collected from all experimental animals (mice, calves and pregnant cows and their offsprings)
vaccinated and control in certain period. The results revealed that all the prepared inactivated rota and corona combined vaccines are potent where they stimulated the development of immune response in all experimental animals by the 2nd week in gel adjuvanted vaccine and 1st week in oil adjuvanted vaccine and these titers increased till reached to its peak and still in high titer for certain times after injection of the second dose of vaccines, then began to decrease gradually
but remained in high protective levels.
Also there was an increase in level of antibodies in sera of newly born calves of vaccinated cows after ingestion of their colostrum.
So we concluded that that all adjuvanted vaccines gave a significant protection to all vaccinated animals, in addition vaccine adjuvanted with oil (Montanide ISA206) was particularly better and effective than gel and oil (IMS 1113).