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العنوان
Isolation and characterization of G10 serotype of group A rotavirus from camels /
المؤلف
Abohatab, Eman Mohamed Mohamed.
هيئة الاعداد
باحث / إيمان محمد محمـــــــــد ابوحطب
مشرف / محمــــــــــد سامي صابر
مشرف / حسيــــن علــــي حسيـــن
مشرف / هادية عبد الرحيم علي موسي
الموضوع
Camels. Rotaviruses.
تاريخ النشر
2008.
عدد الصفحات
170 Leaves :
اللغة
الإنجليزية
الدرجة
الدكتوراه
التخصص
البيطري
الناشر
تاريخ الإجازة
1/1/2008
مكان الإجازة
جامعة القاهرة - كلية الطب البيطري - Virology
الفهرس
Only 14 pages are availabe for public view

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from 193

Abstract

G10 serotype of group A rotaviruses was isolated and characterized from fecal samples collected from camel with diarrhea in farms at Alexandria and Esmalia governorates after preparation of feacl samples for virus isolation and propagation on MA 104 cell line for five passages, two isolates were successfully isolated with a clear and reproducible CPE on the inoculated cells. The isolates were identified antigenically using antigen capture ELISA based on monoclonal antibodies that able to detect any group A rotavirus. Viral RNA was extracted from the tissue culture harvest of the propagated viruses.RT-PCR using primers specific for VP6 and VP7 of group A rotaviruses was employed and confirmed the molecular characterization of the isolates Revealing the correct and expected bands. Amplification of the VP7 gene in the extracted RNA was conducted using PCR and the positive bands were eluted from the agare gel and sequenced using VP7 specific primers sequence results indicated that both isolates had Maximum identity that both isolates had Maximum idenntly to the G10 serotype of group A bovine rotaviruses ranging from 90-93%.This is the first report on the circulation of G10serotype of group A rotaviruses in camel.