الفهرس 
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Abstract
The local isolate of BHV-1 in Egypt was characterized in MDBK cell line for three passages. The isolate was successfully propagated in MDBK cell with a clear and reproducible CPE on the inoculated cells. The 3rd passage was titrated in MDBK cells, and was identified by Electron microscope negative stain technique which gave characteristic morphological appearance of bovine BHV-1.The BHV-1was identified by using IPST, DFAT, VNT, and was identified antigenically. The viral DNA was extracted from the tissue culture harvest of the propagated virus PCR using primers specific for gp C (III) the BHV-1 virus was employed and confirmed the molecular characterization of the isolate revealing the correct and expected bands. After electrophoresis of the PCR. The PCR products was sequenced using forward gp III specific primers sequence results indicated that the local isolate had Maximum identity to the BHV-1.1 by 99%.