الفهرس | Only 14 pages are availabe for public view |
Abstract In this study, 5 batches of inactivated RVF vaccine (ZH501) were evaluated according to DIE RVF evaluation protocol through the following tests: 1- Sterility test, it was done through culturing the tested vaccine on specific media for aerobic, anaerobic bacteria, fungal and mycoplasma. 2- safety tests, to confirm the validity of the vaccine for animal used without any post vaccinal reaction, through both vaccination of 10 mice (3-5 days old) with 0.2 ml of tested vaccine then follow up for 10 days and vaccination of 2 lambs with 5ml SIC and 5ml lIP then daily recording of the body temperature for 10 days. 3- potency’ tests, to determine the duration of vaccinated animal immunity till reach to the protective level by either by SNT and ELISA through vaccination of 4 sheep with one the field dose 1ml SIC then the sera sample were collected from the vaccinated sheep weekly till 7 week post vaccination (WPV) or by EDso calculation by inoculation of 5 group of 10 mice each of them by one dilution from 1:1 to 1:625 two doses one week apart then challenged with virulent RVF virusl03 to 104 MIPLDso. 4- Using the Rt-PCR to confirm the efficacy of Azeridine as inactivator to RVF virus used in tested vaccine through making RT -PCR on master seed RVF virus as positive control samples, RVF virus after inactivated with azeridine and on the eluted RVF antigen from the tested vaccine. |