الفهرس | Only 14 pages are availabe for public view |
Abstract Comprehensive studies were carried out to investigate a pathological and ultrastructural studies of rabbits experimentally infected with RHDV. Moreover to study the pathogenesis of the disease through studying the early stages of RHDV infection parallel with viral detection in the different cells of different infected organs. Different organs of experimentaly infected rabbits were examined histopathologicaly and liver and lungs were examined for the presence of the virus by Electron microscopy examination and correlation of the presence of the virus with the histopathological observations. A total of 135 rabbits were lm. inoculated by 1 ml. of liver suspeusion of estimated LDso and tissue samples were taken from the liver, lung, kidney, spleen and thymus at different intervals post-inoculation (2, 4, 6, 12, 18, 24, 30, 36, 48, 60 and 72 hours and 4, 5, 6, 8, 10, 12 and 14 days, 18 groups). Histopathological observations revealed that the liver was the first organ to be injured, that the liver was the first organ to be injured, exhibiting a severe hepatitis started 24 h pi and electron microscopy studies revealed electron dense viral particles in both hepatic and Kupffer cells in the liver and phagocytic cells in the lung at 24 h pi. and still present until 12 d pi. The main pathological changes were severe generalized circulatory dysfunction, hyperaemia, congestion and haemorrhages, marked degeneration of parenchymatous tissues, prononnced haemorrhagic pneumonia and extensive distuption of lymphoid tissues. The main hepatic lesions were coagulative necrosis, parenchymal and portal haemorrhages and a constant features were enlarged pale, tan, yellow brown or cooked like liver. Haemorrhages in different organs, especially in lung and kidneys were constant and characteristics. Deaths could be attributed to the general circulatory disorders, severe necrotic hepatitis and extensive pulmonary haemorrhages. |