الفهرس 
AbstractOnly 14 pages are availabe for public view
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Abstract
Cryopreservation could affect spermatozoa in different ways; oxidative damage is one
of these possible ways. The objective in this study was to determine the level of
antioxidant enzymes [glutathione peroxidase (GPx) and the superoxide dismutase
activity (SOD)] in both seminal plasma and spermatozoa. Also, the study aimed to
determine the effect of cryopreservation on the sperm DNA integrity, SOD and the
level of lipid peroxidation in fresh, diluted and frozen/ thawed buffalo spermatozoa.
The DNA fragmentation was described using the neutral comet assay (NCA), which
detects the double strand breaks. The results obtained from this study demonstrated
that DNA fragmentation was observed after cryopreservation of buffalo semen in
addition to a decrease in motility, normal morphology and the reduction in the SOD
activity with increase in the level of lipid peroxidation in the frozen/thawed buffalo
sperm. Also, the results showed that the diluent have a role in the decrease of the lipid
peroxidation product.