الفهرس 
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Abstract
A total of 409 samples were investigated bacteriologically to detect the occurrence of staphylococci among. the diseased animals and human, the highest isolation rate was observed in human samples (36%) followed by dog (28%), bovine (24.8%), ration (14.7%) and chicken (120/0) samples. A total of 78 S. aureus isolates secured from different animals and human origins were characterised and identified using the most important conventional biochemical tests as anaerobic glucose fermentation, catalase, coagulase, acetoin production, novobiocin sensitivity and mannitol fermentation. SpA was extracted from 17 S. aureus isolates (5 bovine, 6 human, 2 dog, 2 concentrated ration and 2 chicken isolates) as well as from Cowan 1 strain to estimate the molecular size of SpA by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) analysis. Antibiogram patterns of 15 S. aureus isolates has been fully studied.