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Abstract This study, was carried y<2)e-s \)e\onging to tbe Hold~ G.~ W1 ~’Ccfi products and vaccines (Y ACSJiMJ). ’LW,fi; imntlmi~aif6-n protoccls used for preparation of polyspecific horse antivenins were evaluated. First and second immunization schedules followed by rest period of about one month ended by reimmunization schedule. The antivenoms were raised to venoms of the four major snakes of South Sinai (three vipers”Cerastes cerastes, Echis coloratus, Pseudocerastes fieldi” and the elapid’ Walterinnesia aegyptia”). In the first schedule, the horses were primed with the individual venoms in subsequent injections at two week intervals. whereas, in the second schedule, the horses were primed by the injection of a mixture of the four venoms. For boosting the immune response, various venom weights were used in the two schedules. The venoms were prepared as indicated for each injection. Generally, the subsequent booster doses from each venom were either constant or increasing. Also venoms emulsified in Complete Freund’s Adjuvant were used in the first four injections of first and second immunization schedules(l-4) and for the reimmunization boosting doses whereas no adjuvant was used in the boosting injections (5-7). Practically, as most of the variables which affect the immunization schedules namely ’the frequency of injections, duration between the successive injections, the type of adjuvant and the venom pool’ were kept constant, the immunization schedules were made different with. respect to the mode of priming and the size of the immunizing doses (as the main variable). |