الفهرس 
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Abstract
The comparative diagnostic evaluation of the six antigens prepared from Sarcocystis fusiformis cysts (whole large-sized [WLC], whole medium-sized [WMC], whole small sized [WSC], cyst wall [CW], cystozoites [CZ] & cyst fluid [CF] by ELISA. The seroprevalencs were 42.9, 47.6, 66.7, 71.4, 100, & 85.7 %, respectively. The highest sensitivity and specificity were achieved by CZ antigen. It reached 100% each. However, the CF showed the lowest specificity 25%. The cross reactivity between S. fusiformis and T. gondii was evaluated and CZ detected the higher seropositive cases. By SDS-PAGE WSC antigen detected 15 protein bands ranged between (14.35-114.9) KDa followed by CZ antigen which detected 13 bands ranged between (18.7-116.6) KDa. WB demonstrated one specific band of 16.7 KDa with CZ and common band of 23 KDa with WLC, WMC and CZ antigens when using naturally infected S. fusiformis buffalo-sera. A commn band of 77KDa, 74 KDa and 143 KDa were demonstrated with WLC & CF, WMC & CW and WSC & CZ antigens, respectively when using T. gondii anti- sera . Moreover, there were 3 common bands of 35, 27& 23 KDa were detected on using CW & CZ antigens against rabbit anti- whole S. fusiformis cysts, rabbit anti-cystozoites & rabbit anti-cyst wall sera, respectively. Also the incidence of S. fusiformis cysts among water buffaloes was redetermined. It was 32.9 %. The highest incidences were recorded in females 93.5 % and in animals aged over 5 years 93.7 %.