الفهرس 
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Abstract
In this study we examine a total number of 160 samples from different sources (diarrhoeic calves, sheep, goats and mastitic cow milk in addition to human stool and urine) the incidence of isolation of Ecoli from animal and human sources reached 23.8%. The results of the serological identification of 38 isolates of E coli from different sources revealed that, only 28 isolates could be typed and grouped under 11 different 0 sero groups (0164:K-, 026:K60, 0126:K71, 0127:K-, 0111:K58, 0157:K-, 086:K61, 025:K-, 0158:K-, 0119:K69, 078:K80) while 10 isolates were untypable by the available anti-sera. We investigated the phenotypic characterization of the isolates for detection of associated virulence character through Hemolytic activity of the isolates, cytopathic effect on the Vero cells and Congo red binding activity. And examine genotypic characterization of the isolated serovars using the PCR (s-PCR and m-PCR) to study the associated virulence genes profiles. Results of detection of hylA (hemolysin) gene of E coli (80.7%), stb (heat stable enterotoxin b) gene (53.6%), Stx1 gene (shiga toxin 1) (3.6%), m-PCR for detection of the LT-I and L T-1/ genes of E coli (7.2%), m-PCR for the detection of the F1? gene (F1? fimbrial) of E coli (67.9%), k99 gene of E coli (50%), sta gene (heat-stable enterotoxin a) of E coli (39.3%), eae gene (intimin) of E coli (10.7%), Results of detection of Flich7 gene, Stx2 gene and F41 gene revealed that all of the tested 28 E coli isolates showing a negative amplification.