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Abstract Mycoplasma infections of small ruminants are known to exist in the Mediterranean region and cause significant economic impacts. Little is known of survivability and control of mycoplasmas under desert conditions. One hundred and twelve (10.890/0) mycoplasmas isolates were obtained from 1028 samples (nasal swabs, tracheal swabs, lung tissues, pleural fluid, ear swabs conjunctival swabs and milk) which were collected from 372 sheep and 127 goats under desert conditions. The rate of mycoplasmas isolation in sheep was 6.87%, while it was 20% in goats. Total mycoplasmas isolates (112) were differentiated by digitonin sensitivity test into 79 isolates (70.540/0) of Mycoplasma species and 33 isolates (29.46%) ofAcholeplasma species. By using growth inhibition test (GIT) for serotyping the 79 Mycoplasma isolates, only 73 isolates were fully identified as follow; Mycoplasma arginini (n = 41), Mycoplasma ovipneumoniae (n = 19), Mycoplasma mycoides subsp. capri (n = 5), Mycoplasma agalactiae (n = 6), Mycoplasma putrefaciens (n = 2). Azythromycin, enrofloxacin and oxytetracyc1in were the most effective antimicrobial agents against ’isolated Mycoplasma species in vitro by using Minimum Inhibitory Concentration test (MIC). from this study, it was proved that ELISA as a serological method is more rapid, simple and sensitive diagnostic technique than traditional culture isolation method in diagnosis of mycoplasma infections, but culture isolation method was important to determine the treatment and control. |