الفهرس 
English coverOnly 14 pages are availabe for public view
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Abstract
A total of 176 samples ( 88 serum and 88 whole blood samples with ethylene diamine tetra acetic acid (EDTA anticoagulant) from Pregnant and non pregnant women with average age (17 -45) years and 172 samples including 86 serum samples (62 sheep and 24 goat) and 86 whole blood samples with EDTA anticoagulant (62 sheep and 24 goat) , were collected from El- Fayoum Governorate . positive percentage of EUSA IgG against T .gondii in pregnant and non pregnant women 30.7 % and 13.7 % respectively .The positive percentage of EUSA IgM against T .gondii in pregnant and non pregnant women 20.5% and 7.95 % respectively. The highest positive percentage of Toxoplasmosis in pregnant and non pregnant women was in group 35-45year old at different three centers, where the population are of low education or bad socioeconomic status ; drinking raw milk and eating undercooked meat or may be attributed to uncontrolled number of stray cats around farms and slaughter houses. The detection of the T.gondii genome in maternal blood with results of a positive ELISA (IgG and IgM), the positive percent of PCR results in examined pregnant and non pregnant 21.5 % and 9 % respectively. When comparing examined pregnant and non pregnant women at large three centers at El-Fayoum Governorate by different serological tests and PCR; it was found that the total positive of EUSA IgG, (44.3% ), EUSA IgM (28.4 %) ,Dye test (21.6) and PCR (30.5 %). The highest positive percent EUSA IgG and PCR were for detection of T.gonciJi. The high sensitivity combined with the high specificity offered by the PCR could actually detect T.gondii in blood specimens of pregnant and non pregnant Women. Comparing the examined serum samples and whole blood samples in sheep at EL- Fayoum Governorate by different serological tests and PCR; total positive ELISA IgG was (98.4% ) Dye test (90.3%) and PCR (67.7 %) .For goat in the same region by the same tests EUSA IgG was (41.7%) , Dye test (20.8%) and PCR (25 %) .The high seroprevalence of T.gondii in these animals may be considered the main source of T.gondii infection to human through consumption of raw or under cooked meat contaminated with tissue cysts of T.gondii.