الفهرس 
AcknowledgementOnly 14 pages are availabe for public view
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Abstract
Twenty-Five samples (aspirate) from swelling of buffalo, 75 lymph nodes samples from sheep were collected and isolated !6 isolates (6 buffalo. 1! C.pseudotuberculosis were identified biochemically, bacteriologically & by PCR for detection PLD gene at 930 bp. Identify of 7 sheep and 7 butTak RAPD-PCR showed great genetic variation could be detected between sheep & buffalo strains and between the isolates of the same specie, the optimal condition and media suitable to maximize PLD of C.pseudotuberculosis productivity were shown that optimal pH was 7.2 and many additives as different ammo acids serum, glucose, eagles media were of low value and the two stages media (cooked meat media) is the most suitable media, for maximizing Pl.D ar investigated media under different conditions were measured by performing (SDS-PAGE electrophoresis for all supemataiits and elccirophoresed gel v, by soft ware, synergistic hacmolytic activity of each supernatant was evaluated by titration method and no correlation could be emphasized between haemolytic titer the actual amount of PLD in supernatant. Three groups of buffalo each contain 3 animals vaccinated with C.F toxoid vaccine, recombmant PLD vaccine control group. The efficiency of vaccines was studied by collecting blood from buffalo after 1 st dose, 2nd dose & challenge and by using ELISA. the titer of antibody showed increase in vaccinated animals with two vaccines and after challenge control group showed clinical symptoms of typical lesions (OSD).