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Abstract A total number of 80 balady sheep, their ages ranged from 3 _ 5 years were included in the present study. Sheep were belonged to Assiut Province specially the following places: 1- Animal production station in El-Hawatka. 2- Assiut Abattoir. 3- veterinary clinic of the faculty of veterinary medicine, Assiut university. 4- Private cases. Animals were classified according to the healthy status after they subjected to careful clinical and laboratory methods of examination into: (l)Clinically healthy group which included 20 sheep. (2) Group I I which suffered from impaction which included 20 sheep. (3)Group III which suffered from tyrnpany and included 20 sheep. (4)Group IV which suffered from indigestion and included 20 sheep. The levels of the following blood serum parameters were estimated: 61 1- Blood serum ,A-amylase ............................. V/l 2- Blood serum lipase ................................ V/l 3- Blood serum total lipids .......................... g/l 4- Blood serum triglyceride .......................... g/l 5- Blood serum cholesterol ........................ mg/dl 6- Blood serum calcium ............................ mg/dl 7- Blood serum glucose ............................ mg/dl 8- Blood serum total protein g/dl The most important clinical signs in examined sheep can be summarized as following: (1) Clinical finding of impaction: Animals suffering from impaction showed transient loss of appetite, reduced ruminal contraction, grey soft faeces, some cases tend to lie down and others showed signs of diarrhoea with yellowish green coloured, sometimes tinged with blood, dull sound is heard by percussion of the rumen. (2) Clinical findings of tympany: Diseased animals showed distension of left side of the left side of the abdomen, animals become” discomfort and may get up and down frequently, dyspnoea associated with protrusion of the tongue, salivation and extension of the head, increased respiratory and pulse rate and tympanic sound is heard by percussion of the rumen. (3) Clinical findings of indigestion: Sheep suffered from indigestion showed signs of dull- ness, suppression of rumination associated with decreased ruminal movement. Faeces contains undigested grains of food. Biochemical analysis of blood serum in examined sheep revealed the following results: (l)A highly significant elevation (p<O.Ol) in the levels of blood serum A-amylase, lipase, total lipids, triglyce¬rides, cholesterol, calcium and glucose values in sheep suffered from impaction when compared with the control group. (2) A highly significant elevation (p<O. 01) in blood serum A-amylase, lipase, total lipids, triglyceride and glucose levels were evident in sheep suffered from tympany were observed. Meanwhile the blood serum total protein and cholesterol levels showed a non significant variation. In addition blood serum calcium levels showed a significant decrease (p<O. 05) in sheep suffered from tympany when compared with control group. (3)A highly significant elevation (p<O.Ol) in the levels of blood serum A-amylase, lipase in sheep suffered from indigestion were evident. (4)A highly significant decrease (p<O.Ol) in the levels of blood serum total protein, calcium, cholesterol, total lipids and triglyceride. Meanwhile there was a significant increase (p<0.05) in blood serum glucose values in sheep suffered from indigestion were observed. It be concluded that evaluation of exocrine pancreatic function test under some aspect of ruminal upset in sheep is a matter of depates, despite the marked increase of lipase and A-amylase activities in serum of diseased sheep it does not mean that the main cause is such disturbances is the pancreatic dysfunction whatever the activities of serum lipase and amylase activities have another origin rather than the pancreas itself exact explanation of relationship between ruminal dystfunction and the increase in pancreatic A-amylase and lipase activities required more attention and further investigation as will. |