الفهرس Only 14 pages are availabe for public view
 |  | from | 157 |  |  |
| | | from | 157 | | |
Abstract
The aim of this work was to study the growth response of S.
persica in vitro via micropropagation and consequently the
production of identical true type plantlets using shoot tip and nodal
cutting explants. Micropropagation of S. persica was achieved by
using shoot tips and nodal cuttings through the starting and rooting
stages. The effect of growth regulators was investigated. The best
medium for shoot tips and nodal cutting was MS basal medium
supplemented with 2.0 mg/l kinetin and 0.1 mg/I IAA. This
balance stimulated 66.6% of shoot tips to form new shoots with
88.9% survival and 11.1 % of them formed callus. The same
balance stimulated 66.6 % of nodal cuttings to form new
shoots with 83.3% survival and about 16.7 % of them could
form callus. Preliminary phytochemical screening identifies the
presence of carbohydrates and/or glycosides, free flavonoids, sterols
and/or triterpenes, alkaloids as well as tannins more strongly with
shoot samples propagated from tissue culture technique indicating
their occurrence in such samples with higher amounts. The UV and
the visible spectra were recorded by the spectrophotometer for the
tested extracts prepared from both the wild and tissue culture
propagated plant of S. persica. The UV/visible spectra of the ethanol
95% extracts of both the wild and tissue culture propagated plant
were similar. The intensities of the absorption bands were higher with
those extracts prepared from the plant propagated by tissue culture
technique and extracted with chloroform, ethanol 70% and absolute.