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Abstract
1. Introduction Nutrition plays an influential role in human health, Meat and meat products contain elements, some of these are constituents and others are added to the product during processing for technological, microbiological or sensory reasons (salt, nitrite, and phosphate).Pastrami is a popular delicatessen meat usually made from beef, and, like corned beef, originally created as a way to preserve meat before modern refrigeration. For pastrami, the raw meat is brined, partly dried, seasoned with various herbs and spices, then smoked and steamed. Although beef navels are the traditional cut of meat for making pastrami, it is now common to see pastrami made from beef brisket, beef round and turkey. 2. Aim Of The Study The purpose of present study is to investigate the effect of eating the dry cured meat indifferent concentrations on the female human health using of White experiment animals in kind (Sprague dawley) 3. Subject And Methods 3.1. Investigated Samples Been obtained for commercial Pasterma (cure – dried meat) were purchased from the local market and divided into four sample with different concentrations. b3.2. Determination Of The Nitrite, Nitrate, And Total Nitrite Content Of The Commercial Pasterma Samples Nitrite and nitrate content and nitrite content were estimated. After that, nitrate content and total (total sodium nitrite) content were calculated. The analyzed showed that the sodium nitrite content of the commercial pasterma samples was from 397.08 – 424.05 mg/kg. 3.3. Pasterma preparation All of pastrami sample were obtained and milled blended it directly with experimental diet formulas. 3.4. Experimental animal Forty were obtained from normal albino rats of Spargue Dawley white female, Weighting 131±2 were obtained and fed on blend basal diet for six consecutive days before starting the experiment for animal adaptation .3.5. Experimental design The rats were divided into 5 groups each with similar total body weight. All the group of rats were fed the experimental diet for 28 days according to the following groups: Studied Groups: A. Control Group: Group (1): (Control negative): Fed on basal diet (casein diet) B. Pasterma Sample Groups: Group (2): Fed on basal diet without plus sample 5% pasterma. Group (3): Fed on basal diet without plus sample 10 % pasterma. Group (4): Fed on basal diet without plus sample 15 % pasterma. Group (5): Fed on basal diet without plus sample 20 % pasterma. 3.6. Biological Evaluation: Biological evaluation of the different diets was carried out by determination of body weight gain % (BWG%) and food efficiency ratio (FER). where there was consensus between the consumption of food and increase body weight gain in rate significantly, especially in groups containing high concentrations. 3.7. Effect Of Pastrami On BWG and FER: As for BWG and FER, the comparison group reported high values as a result of major nutrition-free material transaction treatment is pasterma For the remaining treatment groups it was very noticeable weight gain, where he was on a continuous rise up pasterma concentrations, especially in groups of 10% and 15% and 20%. 3.8. Effect Of Pastrami On Total Body Weight And Organs’ Weight Regarding final weight, the highest value was for group 20%, while sample group 5% showed the lowest value. As for organ’s weight, the results revealed that 4 group 20% showed the highest values of ovary, liver, kidney, lung, spleen, and brain. 3.9. Separation of blood serum: The end of experimental, Animals were sacrificed and blood samples were obtained from the hepatic portal veins then stored at room temperature for 15 minutes. After that samples were centrifuged at 4000 / min, Serum separated in clean glass well – stoppered and stored at(- 20 º C) until analyzed 3.10. Statistical analysis: Statistical analysis were performed by IMB – P – C computer hardware compact 1998, under Windows Microsoft Office 2000 compared with program using statistical package for social Science(SPSS, 2000), and compared with each other using the suitable test. All obtained results were tabulated and suitable recommendation were given. 3.11. Biochemical Analysis: Biochemical analysis was carried out on blood and out on blood and blood serum to determine CBC, serum glucose, Liver function indices, kidney function indices, and serum lipids. 3.12. Organ’s Weight The organs (ovary, liver, kidney, lung, spleen, and brain) were removed and washed in saline solution, weighted and kept in formalin solution (10%)3.13. Histopathological Examination: Specimens from (ovary, liver, kidney, lung, spleen, and brain) were subjected to histopathological examinations 4. Results 4.1. Effect Of Pasterma On Biochemical parameters (CBC, serum glucose, Liver Function Indices, Kidney Function Indices, Serum Proteins, And Serum Lipids). 4.1.1. Complete blood count (CBC) : Regarding Hb, the results clear that the highest value was for control negative, while the lowest value was for 20% group which was significantly less (p < 0.05) compared with control negative group, But groups there similar value. As for RBC, 20% group showed the lowest value which was significantly less (p < 0.05) compared with control group, while the
highest value was for control group and 10% group. For PLT the highest value was for 10% group, whereas control group showed the lowest value. As for WBC, 5% group and 15% group the highest value, while the lowest value was for 10% group. 4.1.2. Serum glucose: For S. glucose, control group showed the lowest value, but the highest value was showed by 5% group. 4.1.3. Liver Function: Regarding S.ALK the highest value was for group 20% pasterma, while control group showed the lowest value. For S.GPT, group 15% the highest value of the other group, where as control negative the lowest value. As for S.GOT the control negative was the highest value, the highest value was for group 20% pasterma. 4.1.4. Kidney Function Indices: Data clear that S. Creatinine, the highest value was for control negative, but the lowest value was for group 10%. Regarding S. Urea, the group 20% won the highest value, the group 5% less than the value of the all group. As for S. Uric acid, the highest value for group 10%, while the lowest value for group 20%. 4.1.5. Serum Lipids: Results was clear that S.VLDL-C, the highest was for group 5%, where as the lowest value for group 15% pasterma. For S.LDL-C control negative was the highest value, but the lowest value for group 5%. As for S. HDL-C, was the highest value for control negative, while the lowest value was group 15% pasterma. Regarding S. Triglyceride, Data revealed that the highest value was for group 20%, while 15% was the lowest value. For S. Cholesterol, control group was the highest value, while group 10% and 20% was the lower value. 4.2. Effect Of Pasterma On Histological Structre Of Ovary, Liver And Kidney. Regarding Ovary, the histopathological examination results revealed that control negative group didn’t show any histopathological alteration. but the result showed mild increased maturation in the group 5%, 10%, 15, and 20pasterma. As for Liver, the histopathological examination results showed that the best result were for control negative groups, 5%, and 10% pasterma, while group 15% pasterma showed mild degenerative changes in hepatocytes, but group 20% pasterma revealed high magnification. For Kidney, the result of the histopathological examination showed that control negative groups showed normal histology of the renal glomerular and tubules, and interstitionil tissue, Also, Kidneys rat from pasterma 5%, 10% diet group showed no histpathological changes. Mean while, kidneys of rat of pasterma 15%, 20 % diet showed mild clawdy swelling degenerative changes in tubules, interstitional tissue and glomerular.