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Abstract
Summary
Diabetes mellitus consider the sixth most common cause of death in 2001, it has a lot of medical and dental complications
Cytokines are low molecular weight glycoproteins produced by immune as well as nonimmune cells.
In diabetes mellitus the release of proinflammatory cytokines (IL-1β , TNF α , IFNγ ) are increased , while inhibitory cytokines (IL-10, IL-11, TGFβ1) are largely suppressed .
Ozone therapy is proving to be a new therapeutic modality with great benefits for patients. Ozonetherapy is today the only approach able to normalize the chronic oxidative stress due to diabetes mellitus.
The aim of the present investigation was to study the effect of alloxan induced diabetes on the gingiva and periodontal ligament of rats under systemic ozone therapy through histological evaluation using hematoxylin & eosin and Masson’s trichrome stains. Immunohistochemical localization of interleukin-1beta using avidin – Biotin peroxidase system with IL-1β mouse monoclonal antibody .
Forty adult male albino rats were obtained with an average body weight 140 grams and divided into 4 equal groups (10 animals each):
-Group I animals served as nondiabetic controls.
-Group II animals received a daily intraperitoneal injection of one cm3 of ozone-oxygen (O3O2) gas mixture with ozone concentration 70 microgram /1cm3 .
-Group III animals received alloxan (uriede of mesoxallic acid) supplied in powder form and dissolved in distilled water to be given as a single intraperitoneal dose of 150 mg/kg body weight for the induction of diabetes mellitus.
-Group IV animals were treated with alloxan to induce diabetes mellitus as in group III then received (O3O2) gas mixture daily for two months in the same dose and route of administration given to group II animals.
The whole experimental period lasted for two months. At the end of the experiment, the animals of the different groups were sacrificed by cervical dislocation; the upper and lower jaws were dissected free and separated into halves.
Gingival specimens were taken from the first molar region of the right halves of the jaws, fixed, washed, processed to be embedded in paraffin , sectioned and stained with hematoxylin & eosin and Masson’s trichrome stains.Other sections were used for immunohistochemical localization of interleukin-1beta using avidin – Biotin peroxidase system with IL-1β mouse monoclonal antibody .
Jaw specimens of the left halves were similarly fixed, washed and decalcified in 10 % EDTA solution.
After complete decalcification, the jaw specimens were washed, processed, embedded in paraffin , sectioned and stained with hematoxylin & eosin and Masson’s trichrome stains.Other sections were used for immunohistochemical localization of interleukin-1beta in the periodontal ligament using avidin – Biotin peroxidase system with IL-1β mouse monoclonal antibody .
The expression of IL-1 β was interpreted as follows:
-ve for negative reactivity.
+ve for weakly positive reactivity.
++ve for moderately positive reactivity.
+++ve for strongly positive reactivity.
Specimens from the pancreas were taken from the animals of the different groups, fixed, processed, embedded in paraffin and sectioned to be stained with hematoxylin and eosin for histological evaluation of the islets of langerhans.