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Abstract -106 - SUMMARY 1- Identification of physiologic races of wheat leaf rust during 1979 - 1981, revealed the existence of races 6, 12, 57, 77, 114 and 144. J”ourty isolates of race 77, which occupied the first rank , were identified during this period. 2- Wheat varieties or lines carring the Lr9, Lr 13, L!-!2, Lx 24 and Lx 2a genes of ~esistance .ad the widest spectrum of resistanqe being .18kly effective to most isolated leaf ~uat races • On the other hand, genes Lx 2d, Lr ~4a, Lx 1, Lr 10, Lr 18, Lr 12, Lx 11, k.2” Lr 14 b and Lx 17 were of minor importance, .ince they were poorly effective against virulent leaf r~st races. J- ~2 monosomic analysis BAowed that two independent genee located on chromosomes LA and 5D controlled resistance of Giza 155 to race 57, wherea~ resistance of Giza 157 to the same race was governed by twod •••• &Dt complement aryl genes, located I on caz-cmoae •• 6 :8. I 4- The two independent genes conditi Ding the reaction of Giza 155 to race 77 were 1 cated on chromosomes 4 B and 6 D, of which chr osom. 4 B carried gene (a) gOTeraing resistance to race, wkereas, ------- L _ -101 .- ch.romosome 6 D was oontIibuting to virulence •.•v.ing probably inhibitor gene(s) for resistance. 5- As concerns Giza 157, chromosome 5 D was found to be involved in controlling leaf rust resistance to race 77. ?urthermore, chromosome 4A was associated with resistance to tbe same race, while minor genes carried on chromosome5A could be suggested t. Aave negative effects on resistance • 6- Higher amounts of sugar contents were observed in all monosomes of Giz~~d Giza 157, showing susce- r. eptible reactionJ3 to races 57 and 77, indicating that susceptibility was characterized by variable degrees of incrmnent in levels of total, reducing and non-reducing sugars. 7- Resistant seedlil~a derived from monosomy lA ef Giza 155, 1•• culated with race 57, contaiRed higher amounts of t.e amino acids Cysteine; Histidine, Arginine and to some extent of Leucine - I•• leuciae taan tlle susceptj.ble ones. 8- Resistant seedlings of monosomy 5D of Giza 155 were c.b.araoter~z.d by tAe presence of ll1g)ler amount er !yrosine, Valille and PheD,yJalanine , abaeace er Proline and 10••• t ’V’slueof Lysine, than tlte IIWIceptible seedli... inooulated with race 51. ----- -- ---- --- -- ---._--- ------- -108 - 9- Resistant seedlings derived from monoSQmy 7A er Giza 157 contained higker amounts of Cysteine and Tyre. _ ine; while those of monosomy 6 B contained kigher amounts of aysteine, Histidine, Tyrosine and Pkenylalanine than tAeir susceptible seedlings. 10- In tests wit. race 77, amino acids analysis indicated taat the resistant seedlings of monosamy 6 D ef Giza .155 were characterized by the presence of k1gker levels of Cysteine, Histidine, Tyrosine and paeuyIalanine; while tke resistant seedlings of monOSQmes 4 A and 5 D of Giza 157 contained higher levels ef Glut- 8.Ilic acid ..T•h.reonine, Alaaine, Tyrosine, Valine , Phenylalanine and Leucine +Isoleucine ae compared with Busceptible seedlings. 11- No marked differences in amounts of total, tree and conjugated phenols were noticed between res1atamt and susceptible Seedlings of monosomy lA of Giza 155, when inoculated with race 57. 12- High levels of total and free phen.Is were detected in the resistant seedlings of mOnoBomy 5 D of Giza 155 carrying genes ot resistance to race 57. 13- ReSistant aeedliQgs of monosomic family 7A of Giza 157 contained lower total and free phenols tJaan t..8uaceptible ones, inooulated with race 57. ~~~ --- --- --- - _. -- - - - -- ..- -- -_. -- .__ .. - - -._---- -’-- - - -----,- - 109 - 14- W~en -iAoculated with race 11, resistant se~~lings at tke family 6D of Giza 155 exhibited higher amounts of tke three forms of phenolic compounds as compared with susceptible seedlings. 15- The effect of resistance genes to race 77 carried on chromosome 5D of Giza 151 was reflected by producing higher levels of total and free pAenols in the resistant combinations. 16- The total and conjugated phenols Were higher in the inoculated resistant seedlings of monosomic family 4A of Giza 151 than in the susceptible seedlings when inoculated with race 71. 17- Peroxidase enzyme activity was higher in susceptible seedlings of all monosomic lines of wheat varieties Giza 155 and Giza 157, inoculated With either race 51 or 77 af leaf, rust. 18- No general trend was observed concerning catalase enzyme activity in resistant and susceptible seedlings of tJae different monosomic lines • .l4onosomes 5D and 6D of Giza 155 which were either inoculated wit. race 57 or 77. ezbib1ted higher activ1t7 in susceptible seedlings tA8D in resistant ones. on tae contrary, monosomefl 7A and 4A of Giza 157 s1lowed lower activity in susceptible than in resistant seedlings. Similar .~tivities were measured in both susceptible and resistant seedlings or monosomic lines 6 Band 5 D of Giza l5~ inoculated witk race, ------------- -110 - 57 and 77, respectively. 19- Susceptible seedlings of all monosomes. inoculated with race 57 exhibited higher activity of ascorbic acid oxidase than resistwlt ones. With regard to race 77, no activity was determined in suseeptible seedlings of all monosomic lines and also in inoculated resistant seedling of monoBomy 5D of Giza 157. 20- No activity of plIly ph eneL oxidase enzyme CQuld be detected in any of mcmosomic lines of Giza 155 and GilSa ~57, when inoculated wi.th either race 57 or . |