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Abstract The experiments included Experimental Farm of Faculty Zagaziig University and at seal-ch Center (NRC) at Giza . in this study weye carried out at t~~e of Agriculture Science at Moshtohor f the Experimental Farm of National Re- TI1C :’~~perinlcnt inclL,lded studying the pYcpag~ticln of Lippia citriodoya using some growth regulators namely IBA and NAA . U~if::~ l”-;Tl Cuttj r:gs f rorn t~-!f:.:’ ’t s-rrm n e l and mi d d Le pl’::,.I”,’t pC!i·ti.;:~;r-·, ’”·.’C~l-E ::’:’dC’” .J’~”:~’:”::’;::; t::: ci ffc’rer:t: I_;’·c.c’,:n.c-”-.t:c. of IBt, ’:.0.c: ~ 150 f 25~:~ ,:,’( 350 FPt’1 f in \-y’Z!,tE·r col ut ior, z,nd C~O , 1500 , 2500 and 3500 F’PMin t,,~,lc pO~·,I-- dEY~ and NAA (0.0 ,50 1r-(1 or 200 PPM in watEr solution and 0.0 , 500 , 1000 , 2000 F’F’Min t ,;;,1 c pOI,,·~lder) MClyeOVEr field experiments were performed in the two season to ~3tudy the effects cof t~1e location factors and seasonal variation on the growth, essential oil production and the oil constituents The effect of frequent harvesting on the yield of leaves and twigs , oil production and oil constituents was studied as well as the effect of foliar nutrition, using ”Foliatrian’l in four concentrations as 0,2,3 and 4 mIlL was included. Lippia citriodc’Y2 oil was subjected to GLC, GCapillary CG analysis and G2~ c~lYomatc,;YaphY-~25S spectc,metry GC-MS an51ysis Besides studying the effect of extraction metJ10ds on the percent2gc clf oil and oil cc,nstitL’e~ts ~as done. The most important results were T __ EFFEI-:T OF 13F.:Ol,.-JTH F-~FC:iUL~T;OF:~; A!\!D TYPES OF CUTT I rJ(3S ON THE PonT Tt-·,~(; PESPONSE • cr e a s e d T~,e application of lEA the rooting pE’j-CentO::::ige of 150 and 250 PPM significantly Lippia citriodora . i n-- TIle middle pOl-tion cuttings treated with lBA at 250 PPM gave the best rEsults of rooting and significantly irlcreased t}lS root length . IDA was more EffectivE on rooting of Lippia citriodora cuttings 2S compared to NAA Especially when middle portion cuttirlg WGYE used as when the growth regulators was used in aq~ous solution • 2 In all cases, the middle ~~~-tiorl cuttings of Lippia cj,tri odora rooted mort than terminal cuttings 3- Generally application of growth regulators (lBA and NAA) in talc powder had slightly increased the percentage of rooted cuttings::. , I I ~ 1-- EFFE’C’:T OF LDCATION FAC:TOF:~S :”1N THE C:.iPDWTJ-,1 f\ND OIL. F’F:DDL1CTTDr>J O~ LIPPIA CITRIODORA . Ttl0 cC’nditions of ~1osflt0hc~r lOC2tlO~j were favorable in compari- 5 (-:’;’-i te, C:i:~2. ~’:;cz:.tic:’~·; sir’,::€-: ·I..t ;:~gh vc.I. ..:::’:”’~’:.<:..:;~ , pl,;:\nt l”ic::i£,:ht , nUfi~teY ~f branche~ plant leaf &rE2/~lant and frc anci C!lP / yield of leaves 2nd twigs ~JEYC recorded for MOS.ltohor plants cc,mpared with Gi~a plants ~ On t~~e ether hand t ~h~plants growth at Giza gave hig~ler values of number of leaves per ’plar,t • r ; At full blooming stage (on S~ptember) , the higher percentage of Luisa oil was obtained fr-om thE’ j:.,li:.”1nt.,s. g~~own undf?,( the corrd i rtions of l:ilZ2t location whETeasi the highest oil yield ””’1as:. obtained from Giza location when the plants werE harvEsted in SeptembEr ~ 11.2. EFFECT OF SEASONAL VARIATION AND LOCATION ON THE YIELD OF LEAVES AND TWIGS , OIL PRODUCTION AND OIL CONSTITUENTS 1- The maximum fresh and dry weights of leaves and twigs was r-ecCtl”-ded during Oc tobe r at. Moshtoho’( i r: (Septeinb2’r-) 2t 13izl3.-, Plants at Moshtohor and twigs compared with produced the hEaviest l3izi::1. location ~ of Irl both fresh and dr)! weigh~ year old plants ttlen of 1eaves arid twigs were maximum wit!1 the one declinEd with the two year old plants ~ 4- There was gradual marked decrease in the oil percentage with t.le increased growt~l rate of leaves and twigs. The percentage of oil reached its peak at the early stage of growth in the two locations In all cases plants at Giza location .1ad high oil percentage than those in Moshtohor Comparing the rEsults at the top oil yield production i~ two locations , t~le plants of Moshtohor in both seasons gave t~E hig.lest yield of oil compared with Giza location on 6- ConCErning the main of , t~le effect of seasonal variation and location oi I cc,nstituents UndeF the env.ir::!nmental corid i t ione ent=:- weYE’ 1oc,,:-\ t i c,n • elf MoshtohoY , tf1e highest values obtained on October, while it of tc,tal main constitllwas on November at Giza III EFFECT OF FREQUENT HARVESTING ON THE YIELD OF LEAVES AND T~·!I CS 0 I L PPODUCT I ON AND elL CO!’-~ETI TI !ENTS tE.ined The ’.•>hen higher frEsh and dry yield of the plants were harvested once and .-, The highest ,:”,il yi eld .l-l’ K£:; ,·-.’as ob’tC’tinc:-:·d whE,n thE’ j::;lant~:;. were h2~vcsted 3 t12~ve~ts compaYE’,rj ””lith mer”lt • 13.3 2.nd 10.0 per f e dd en fi ’(st dnd t<g/ fed dan recQrded 18.1 Vg/feddarl and second season respectively f~,y the c:rlE ~laYVest trea”l- :J... 1T:E’ t~j::)tE\J. cil yield 15.6 ~:g!feddar1 d~l-ing the 4- In senera} , frequent harvesting declined the total main constituen,:fs. of vol eti Le oil (Limonc;:e ~ l,E: C:in’::;,”’:ll and Citral) I~}- EFFECT OF FOLIAR NUTRITION ON THE GROWTH? OIL YIELD ANn OI~ CC1l\.1ST I TUE\!TS 1- Foliar spray with Foliatrin at the low level treatment 2 mIll resulted in significant increase in the plant t,eight and frest”\ and dry weig~lts of leaves and twigs compared with other treatments ’-:’-” T~le application of high level of on increasinq t~le pCYCent2ge of the Foliatrian was very effect i ”·,n2 total cf main constituents of vLat i Le- oiI V-ANALYSIS OF VOLATILE OIL The essential oil , obtained after steam distillation of the leaves and twigs of Lippia citriodor3 after; separation on a Silica gel column , was investigated by means of GLC/MS . 1- The oil consisted of about 15.81% -Pinene , B-pinene , D-Limonenc t~epresented thE monotet~penes .. hyd~ocaybon monotrepene3 -Terpinene and Decan 2 Longipinene -Cubeben, -Cedrene, and trans , Far2nsene were identified as .’.ydroc2rbons in Lippia citriodorB oil Humulene , Curcumene a new sesequiterpene 3- 1,8 Cineol and Citral (a+b) were major oxygenated-compounds The oil of Lippia citriodor2 contained also Linallol , Ter~ineol 1 Bornecil B-citronellal GeY2niol Decane 2 one Benzcnc--l-(1,5 dimethyle -4-hexnyl -4--mct~lyl), CaYyop~·illcne oxide &nd 6-Met!1yle-5-hepterl 2 one as a new components in Lippia citriodc1ra oil ’v’I EFFECT OF EXTPl’<\r:T I ON l”’1ETHQDS nl”~ THE=’” PEF::CEf\.!Tt:J:iE p-.ND O! L cONSTITUENTS OF LIPPIA C:TRIonQR~ 1- Maximum of oil percentage was obtained when the fresh leaves and twigs was subjected to steam distillation compared witt, solvent extraction metl10d .” 2 Citral (a~’b) is the ”lajor constitLlents in oils obtained by steam distillation followed by D-Limonene and 1,8 Ciniol , while in -Eibs.;olute oil e:.<tr”acted fyom ::or!cr-ete f (:it:YEll (cl+b) i~· thE m.r::,.jor- ,:onstituents followed by B-CitFonellol and C21~yophyllene oxi~c . |