الفهرس 
Material and methodsOnly 14 pages are availabe for public view
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Abstract
The experiments included
Experimental Farm of Faculty
Zagaziig University and at
seal-ch Center (NRC) at Giza .
in this study weye carried out at t~~e
of Agriculture Science at Moshtohor f
the Experimental Farm of National Re-
TI1C :’~~perinlcnt inclL,lded studying the pYcpag~ticln of Lippia
citriodoya using some growth regulators namely IBA and NAA . U~if::~
l”-;Tl Cuttj r:gs f rorn t~-!f:.:’ ’t s-rrm n e l and mi d d Le pl’::,.I”,’t pC!i·ti.;:~;r-·, ’”·.’C~l-E ::’:’dC’”
.J’~”:~’:”::’;::; t::: ci ffc’rer:t: I_;’·c.c’,:n.c-”-.t:c. of IBt, ’:.0.c: ~ 150 f 25~:~ ,:,’( 350 FPt’1 f
in \-y’Z!,tE·r col ut ior, z,nd C~O , 1500 , 2500 and 3500 F’PMin t,,~,lc pO~·,I--
dEY~ and NAA (0.0 ,50 1r-(1 or 200 PPM in watEr solution and 0.0 ,
500 , 1000 , 2000 F’F’Min t ,;;,1 c pOI,,·~lder)
MClyeOVEr field experiments were performed in the two season to
~3tudy the effects cof t~1e location factors and seasonal variation on
the growth, essential oil production and the oil constituents
The effect of frequent harvesting on the yield of leaves and
twigs , oil production and oil constituents was studied as well as
the effect of foliar nutrition, using ”Foliatrian’l in four concentrations
as 0,2,3 and 4 mIlL was included.
Lippia citriodc’Y2 oil was subjected to GLC, GCapillary CG
analysis and G2~ c~lYomatc,;YaphY-~25S spectc,metry GC-MS an51ysis
Besides studying the effect of extraction metJ10ds on the percent2gc
clf oil and oil cc,nstitL’e~ts ~as done.
The most important results were
T __ EFFEI-:T OF 13F.:Ol,.-JTH F-~FC:iUL~T;OF:~; A!\!D TYPES OF CUTT I rJ(3S ON THE PonT Tt-·,~(;
PESPONSE •
cr e a s e d
T~,e application of lEA
the rooting pE’j-CentO::::ige of
150 and 250 PPM significantly
Lippia citriodora .
i n--
TIle middle pOl-tion cuttings treated with lBA at 250 PPM gave
the best rEsults of rooting and significantly irlcreased t}lS root
length .
IDA was more EffectivE on rooting of Lippia citriodora cuttings
2S compared to NAA Especially when middle portion cuttirlg WGYE used
as when the growth regulators was used in aq~ous solution •
2 In all cases, the middle ~~~-tiorl cuttings of Lippia cj,tri
odora rooted mort than terminal cuttings
3- Generally application of growth regulators (lBA and NAA) in
talc powder had slightly increased the percentage of rooted cuttings::.
,
I I ~ 1-- EFFE’C’:T OF LDCATION FAC:TOF:~S :”1N THE C:.iPDWTJ-,1 f\ND OIL. F’F:DDL1CTTDr>J
O~ LIPPIA CITRIODORA .
Ttl0 cC’nditions of ~1osflt0hc~r lOC2tlO~j were favorable in compari-
5 (-:’;’-i te, C:i:~2. ~’:;cz:.tic:’~·; sir’,::€-: ·I..t ;:~gh vc.I. ..:::’:”’~’:.<:..:;~ , pl,;:\nt l”ic::i£,:ht ,
nUfi~teY ~f branche~ plant leaf &rE2/~lant and frc anci C!lP
/
yield of leaves 2nd twigs ~JEYC recorded for MOS.ltohor plants cc,mpared
with Gi~a plants ~ On t~~e ether hand t ~h~plants growth at
Giza gave hig~ler values of number of leaves per ’plar,t •
r ; At full blooming stage (on S~ptember) , the higher percentage
of Luisa oil was obtained fr-om thE’ j:.,li:.”1nt.,s. g~~own undf?,( the corrd i rtions
of l:ilZ2t location whETeasi the highest oil yield ””’1as:. obtained
from Giza location when the plants werE harvEsted in SeptembEr ~
11.2. EFFECT OF SEASONAL VARIATION AND LOCATION ON THE YIELD OF
LEAVES AND TWIGS , OIL PRODUCTION AND OIL CONSTITUENTS
1- The maximum fresh and dry weights of leaves and twigs was
r-ecCtl”-ded during Oc tobe r at. Moshtoho’( i r: (Septeinb2’r-) 2t 13izl3.-, Plants at Moshtohor
and twigs compared with
produced the hEaviest
l3izi::1. location ~
of
Irl both fresh and dr)! weigh~
year old plants ttlen
of 1eaves arid
twigs were maximum wit!1 the one declinEd with
the two year old plants ~
4- There was gradual marked decrease in the oil percentage with
t.le increased growt~l rate of leaves and twigs. The percentage of
oil reached its peak at the early stage of growth in the two locations
In all cases plants at Giza location .1ad high oil percentage
than those in Moshtohor
Comparing the rEsults at the top oil yield production i~ two
locations , t~le plants of Moshtohor in both seasons gave t~E hig.lest
yield of oil compared with Giza location
on
6- ConCErning
the main of
, t~le effect of seasonal variation and location
oi I cc,nstituents UndeF the env.ir::!nmental
corid i t ione
ent=:- weYE’
1oc,,:-\ t i c,n •
elf MoshtohoY , tf1e highest values
obtained on October, while it
of tc,tal main constitllwas
on November at Giza
III EFFECT OF FREQUENT HARVESTING ON THE YIELD OF LEAVES AND
T~·!I CS 0 I L PPODUCT I ON AND elL CO!’-~ETI TI !ENTS
tE.ined
The
’.•>hen
higher frEsh and dry yield of
the plants were harvested once
and
.-, The highest ,:”,il yi eld .l-l’ K£:; ,·-.’as ob’tC’tinc:-:·d whE,n thE’ j::;lant~:;.
were h2~vcsted 3 t12~ve~ts
compaYE’,rj ””lith
mer”lt •
13.3 2.nd 10.0
per f e dd en
fi ’(st dnd
t<g/ fed dan
recQrded 18.1 Vg/feddarl and
second season respectively
f~,y the c:rlE ~laYVest trea”l-
:J... 1T:E’ t~j::)tE\J. cil yield
15.6 ~:g!feddar1 d~l-ing the
4- In senera} , frequent harvesting declined the total main
constituen,:fs. of vol eti Le oil (Limonc;:e ~ l,E: C:in’::;,”’:ll and Citral)
I~}- EFFECT OF FOLIAR NUTRITION ON THE GROWTH? OIL YIELD ANn OI~
CC1l\.1ST I TUE\!TS
1- Foliar spray with Foliatrin at the low level treatment 2
mIll resulted in significant increase in the plant t,eight and frest”\
and dry weig~lts of leaves and twigs compared with other treatments
’-:’-” T~le application of high level of
on increasinq t~le pCYCent2ge of the
Foliatrian was very effect
i ”·,n2 total cf main constituents
of vLat i Le- oiI
V-ANALYSIS OF VOLATILE OIL
The essential oil , obtained after steam distillation of the
leaves and twigs of Lippia citriodor3 after; separation on a Silica
gel column , was investigated by means of GLC/MS .
1- The oil consisted of about 15.81%
-Pinene , B-pinene , D-Limonenc
t~epresented thE monotet~penes ..
hyd~ocaybon monotrepene3
-Terpinene and Decan
2 Longipinene -Cubeben, -Cedrene,
and trans , Far2nsene were identified as
.’.ydroc2rbons in Lippia citriodorB oil
Humulene , Curcumene
a new sesequiterpene
3- 1,8 Cineol and Citral (a+b) were major oxygenated-compounds
The oil of Lippia citriodor2 contained also Linallol , Ter~ineol 1
Bornecil B-citronellal GeY2niol Decane 2 one Benzcnc--l-(1,5
dimethyle -4-hexnyl -4--mct~lyl), CaYyop~·illcne oxide &nd
6-Met!1yle-5-hepterl 2 one as a new components in Lippia citriodc1ra
oil
’v’I EFFECT OF EXTPl’<\r:T I ON l”’1ETHQDS nl”~ THE=’” PEF::CEf\.!Tt:J:iE p-.ND O! L
cONSTITUENTS OF LIPPIA C:TRIonQR~
1- Maximum of oil percentage was obtained when the fresh leaves
and twigs was subjected to steam distillation compared witt, solvent
extraction metl10d .”
2 Citral (a~’b) is the ”lajor constitLlents in oils obtained by
steam distillation followed by D-Limonene and 1,8 Ciniol , while in
-Eibs.;olute oil e:.<tr”acted fyom ::or!cr-ete f (:it:YEll (cl+b) i~· thE m.r::,.jor-
,:onstituents followed by B-CitFonellol and C21~yophyllene oxi~c .